Publications by authors named "Laura J Musgrove"
Ubiquitin-dependent unfolding of the CMG helicase by VCP/p97 is required to terminate DNA replication. Other replisome components are not processed in the same fashion, suggesting that additional mechanisms underlie replication protein turnover. Here, we identify replisome factor interactions with a protein complex composed of AAA+ ATPases SPATA5-SPATA5L1 together with heterodimeric partners C1orf109-CINP (55LCC).
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- The BRCA1-BARD1 complex is essential for repairing DNA double-strand breaks in the S/G2 phase of the cell cycle by associating with chromatin.
- The research explored how this complex interacts with modified nucleosomes, finding that it prefers di-nucleosomes and can effectively integrate various chromatin modifications.
- High-speed atomic force microscopy revealed that BRCA1-BARD1 forms a dynamic structure that links two nucleosomes, enhancing its enzymatic activity through multiple interactions, even in the presence of marks that typically inhibit its binding.
Membrane proteins are essential for cellular growth, signalling and homeostasis, making up a large proportion of therapeutic targets. However, the necessity for a solubilising agent to extract them from the membrane creates challenges in their structural and functional study. Although amphipols have been very effective for single-particle electron cryo-microscopy (cryoEM) and mass spectrometry, they rely on initial detergent extraction before exchange into the amphipol environment.
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