Length-Dependent Cellular Internalization of Nanobody-Functionalized Poly(2-oxazoline) Nanorods.

The ability to target specific tissues and to be internalized by cells is critical for successful nanoparticle-based targeted drug delivery. Here, we combined "stealthy" rod-shaped poly(2-oxazoline) (POx) nanoparticles of different lengths with a cancer marker targeting nanobody and a fluorescent cell internalization sensor via a heat-induced living crystallization-driven self-assembly (CDSA) strategy. A significant increase in association and uptake driven by nanobody-receptor interactions was observed alongside nanorod-length-dependent kinetics.

Towards Solving the PFAS Problem: The Potential Role of Metal-Organic Frameworks.

Per- and polyfluoroalkyl substances (PFAS) are a group of recalcitrant molecules that have been used since the 1940s in a variety of applications. They are now linked to a host of negative health outcomes and are extremely resistant to degradation under environmental conditions. Currently, membrane technologies or adsorbents are used to remediate contaminated water.

It's what's on the inside that counts: Techniques for investigating the uptake and recycling of nanoparticles and proteins in cells.

Many applications of nanomedicines depend on the therapeutic gaining access to the interior of cells. As most proteins and nanoparticles are taken up by endocytosis, determining the properties of nanoparticles that govern uptake is essential. In this review, we examine the current approaches for measuring the cellular uptake of nanoparticles and proteins.

A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells.

Intracellular trafficking governs receptor signaling, pathogenesis, immune responses and fate of nanomedicines. These processes are typically tracked by observing colocalization of fluorescent markers using confocal microscopy. However, this method is low throughput, limited by the resolution of microscopy, and can miss fleeting interactions.

Silicon-Nanotube-Mediated Intracellular Delivery Enables Ex Vivo Gene Editing.

Engineered nano-bio cellular interfaces driven by vertical nanostructured materials are set to spur transformative progress in modulating cellular processes and interrogations. In particular, the intracellular delivery-a core concept in fundamental and translational biomedical research-holds great promise for developing novel cell therapies based on gene modification. This study demonstrates the development of a mechanotransfection platform comprising vertically aligned silicon nanotube (VA-SiNT) arrays for ex vivo gene editing.