Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH).

The opportunity to map genes and noncoding DNA sequences on the chromosomes by fluorescence in situ hybridization (FISH) has greatly enhanced the potential for fish karyotyping and comparative cytogenetics. The use of FISH allowed for significant advances in our understanding of the fish genome architecture, especially when applied to the study of the repetitive component of the genome, that is generally underestimated in the bioinformatic assembly. Here we provide a step-by-step protocol for FISH of repeated sequences onto chromosomes of fish species.

The Arrangement of the Peripheral Olfactory System of : A Well-Exploited Small Sensor, an Aided Water Flow, and a Prominent Effort in Primary Signal Elaboration.

The olfactory system is constituted in a consistent way across vertebrates. Nasal structures allow water/air to enter an olfactory cavity, conveying the odorants to a sensory surface. There, the olfactory neurons form, with their axons, a sensory nerve projecting to the telencephalic zone-named the olfactory bulb.

Latitudinal Cline in Chromosome Numbers of Ice Cod (Gadidae) from Northeast Greenland.

The ice cod (Peters, 1872) is one of the few fish species endemic to the Arctic. With a circumpolar distribution, the species is confined to the fjords and shelves of the Arctic seas. Biological information on is scarce, with genomic information restricted to microsatellites.

Quantification of neurons in the olfactory bulb of the catsharks Scyliorhinus canicula (Linnaeus, 1758) and Galeus melastomus (Rafinesque, 1810).

In vertebrates, the olfactory bulb (OB) is the zone of the brain devoted to receiving the olfactory stimuli. The size of the OB relative to the size of the brain has been positively correlated to a good olfactory capability but, recently, this correlation was questioned after new investigation techniques were developed. Among them, the isotropic fractionator allows to estimate the number of neurons and non-neurons in a given portion of nervous tissue.

Cytogenetic characterization of the Antarctic silverfish Pleuragramma antarctica (Boulenger 1902) through analysis of mitotic chromosomes from early larvae.

This paper describes the cytogenetic features of the Antarctic silverfish Pleuragramma antarctica (Boulenger 1902), a keystone species of the Antarctic coastal marine ecosystem. Conventional cytogenetic analyses and physical mapping of repetitive DNA sequences were performed on metaphase plates obtained through direct chromosome preparation from P. antarctica early larvae.

Secondary Folds Contribute Significantly to the Total Surface Area in the Olfactory Organ of Chondrichthyes.

The olfactory organ of Chondrichthyes is characterized by a central support with several lamellae covered by a sensory olfactory epithelium. Although secondary folds are present on the lamellae in all the chondrichthyan species analyzed to date, their shape and size have not been described. We here analyze the olfactory organ of 13 elasmobranch and 1 holocephalan species, describe the shape of the secondary folds and evaluate how they contribute to the epithelial surface area.

Insertion Hot Spots of Retrotransposon and Chromosomal Diversifications among the Antarctic Teleosts Nototheniidae.

By their faculty to transpose, transposable elements are known to play a key role in eukaryote genomes, impacting both their structuration and remodeling. Their integration in targeted sites may lead to recombination mechanisms involved in chromosomal rearrangements. The Antarctic fish family Nototheniidae went through several waves of species radiations.

Microplastics in the Arctic: A case study with sub-surface water and fish samples off Northeast Greenland.

The Arctic is a unique and fragile ecosystem that needs to be preserved and protected. Despite its remoteness, plastic pollution has been documented in this region. In the coming years, it is likely to worsen since, with climate changes and the opening of new shipping routes, the human presence is going to increase in the whole area.

Reconstruction of the repetitive antifreeze glycoprotein genomic loci in the cold-water gadids Boreogadus saida and Microgadus tomcod.

Antifreeze glycoproteins (AFGPs) are a novel evolutionary innovation in members of the northern cod fish family (Gadidae), crucial in preventing death from inoculative freezing by environmental ice in their frigid Arctic and sub-Arctic habitats. However, the genomic origin and molecular mechanism of evolution of this novel life-saving adaptive genetic trait remained to be definitively determined. To this end, we constructed large insert genomic DNA BAC (bacterial artificial chromosome) libraries for two AFGP-bearing gadids, the high-Arctic polar cod Boreogadus saida and the cold-temperate Atlantic tomcod Microgadus tomcod, to isolate and sequence their AFGP genomic regions for fine resolution evolutionary analyses.

Clarification of the Terminology of the Olfactory Lamellae in Chondrichthyes.

Several papers regard the anatomy of the peripheral olfactory organ, the olfactory rosette, in the class of Chondrichtyes. The complex shape of this organ and the differences among species give clues to functional, evolutionary, and ecological observations; data on a larger number of species are needed in order to have a more complete insight. The rosette is made up of a central support and of numerous lamellae, which are lined by the sensory epithelium.

Evolution in an extreme environment: developmental biases and phenotypic integration in the adaptive radiation of antarctic notothenioids.

Background: Over the past 40 million years water temperatures have dramatically dropped in the Southern Ocean, which has led to the local extinction of most nearshore fish lineages. The evolution of antifreeze glycoproteins in notothenioids, however, enabled these ancestrally benthic fishes to survive and adapt as temperatures reached the freezing point of seawater (-1.86 °C).

A demonstration of nesting in two antarctic icefish (genus Chionodraco) using a fin dimorphism analysis and ex situ videos.

Visual observations and videos of Chionodraco hamatus icefish at the "Acquario di Genova" and histological analyses of congeneric species C. hamatus and C. rastrospinosus adults sampled in the field provided new anatomical and behavioral information on the reproductive biology of these white blooded species that are endemic to the High-Antarctic region.

Induction of meiotic gynogenesis in Atlantic cod (Gadus morhua L.) through pressure shock.

The Atlantic cod, Gadus morhua, is one of the most important species for commercial fisheries and a promising candidate for aquaculture. Precocious sexual maturation of males is one of the major issues compromising large scale production. The potential approaches to this problem include production of all female populations.

Assembly of the antifreeze glycoprotein/trypsinogen-like protease genomic locus in the Antarctic toothfish Dissostichus mawsoni (Norman).

To investigate the genomic architecture underlying the quintessential adaptive phenotype, antifreeze glycoprotein (AFGP) that enables Antarctic notothenioid survival in the frigid Southern Ocean, we isolated the AFGP genomic locus from a bacterial artificial chromosome library for Dissostichus mawsoni. Through extensive shotgun sequencing of pertinent clones and sequence assembly verifications, we reconstructed the highly repetitive AFGP genomic locus. The locus comprises two haplotypes of different lengths (363.

Cell proliferation and apoptosis in the olfactory epithelium of the shark Scyliorhinus canicula.

To date, no study has been published on cell renewal in the olfactory epithelium of Chondrichthyes. Our work aimed at detecting proliferating cells (by Proliferating Cell Nuclear Antigen - PCNA immunohistochemistry) and apoptotic cells (by terminal uridine deoxynucleotidyl transferase nick end labeling method) in the olfactory epithelium of the shark Scyliorhinus canicula. PCNA immunoreactivity and mitotic figures were localized almost exclusively at the basal and apical thirds of the epithelial thickness.

Ribosomal genes in notothenioid fishes: Focus on the chromosomal organisation.

This mini-review makes a survey and a summary of some major issues concerning the chromosomal organisation of ribosomal genes in fish genomes, by using Notothenioidei as the model. The increasing body of information, published during the last two decades on the chromosomal mapping of the two ribosomal genes classes (45S rDNA and 5S rDNA) in notothenioids, makes it possible to recognise the main evolutionary trends across the phylogeny of the group. As one of the major features, the rDNA clusters are organised in a single chromosomal locus in most of the species.

Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish.

The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci.

Mapping of alpha- and beta-globin genes on Antarctic fish chromosomes by fluorescence in-situ hybridization.

The pathways and mechanisms of genomic change that have led to the peculiar haemoglobinless phenotype of the white-blooded Antarctic icefishes (16 species in the family Channichthyidae) constitute an important model for understanding the rapid diversification of the Antarctic notothenioid fish flock. To provide complementary structural information on genomic change at globin-gene loci in Antarctic fish species, cytogenetic studies and in-situ chromosomal mapping have been undertaken. Using a DNA probe containing one alpha- and one beta-globin gene from the embryonic/juvenile globin gene cluster of the red-blooded species Notothenia coriiceps, we mapped the cluster on the chromosomes of Antarctic teleosts by fluorescence in-situ hybridization.