Nutrition programs enhance exercise effects on body composition and resting blood pressure.

The purpose of our study was to examine the effects of exercise alone and exercise combined with specific nutrition programs on body composition and resting blood pressure rate. Adult participants (99 women, 22 men; aged 20-86 years) completed a combined strength and endurance exercise program (Exercise Only), or in conjunction with 1 of 2 nutrition plans (Exercise/Protein; Exercise/Protein/Diet). The Exercise-Only group performed 1 set of 9 resistance machines regimens interspersed with 3 bouts of recumbent cycling (5 minutes each).

Biochemical and mutagenic analysis of I-CreII reveals distinct but important roles for both the H-N-H and GIY-YIG motifs.

Homing endonucleases typically contain one of four conserved catalytic motifs, and other elements that confer tight DNA binding. I-CreII, which catalyzes homing of the Cr.psbA4 intron, is unusual in containing two potential catalytic motifs, H-N-H and GIY-YIG.

Expression, purification, and biochemical characterization of the intron-encoded endonuclease, I-CreII.

The ORF of the Cr.psbA4 intron of Chlamydomonas reinhardtii mediates efficient intron homing, and contains an H-N-H and possibly a GIY-YIG motif. The ORF was over-expressed in Escherichia coli without non-native amino acids, but was mostly insoluble.