Experimental Growth Conditions affect Direct and Indirect Defences in two Cotton Species.

Cotton has been used as a model plant to study direct and indirect plant defence against herbivorous insects. However, the plant growing conditions could have an important effect on the outcome of such plant defence studies. We examined how common experimental growth conditions influence constitutive and inducible defences in two species of cotton, Gossypium hirsutum and G.

Improved separation by at-column dilution in preparative hydrophilic interaction chromatography.

We evaluated at-column dilution (ACD) as a possible approach to reduce peak distortion caused by the injection of strong solvents in preparative hydrophilic interaction chromatography (HILIC). Three model compounds, namely uridine, cytidine and guanosine were selected and injected either in conventional or ACD conditions, using various proportions of water and acetonitrile in the sample diluent. Plate number and peak capacity were systematically investigated under both isocratic and gradient elution conditions, respectively.