Publications by authors named "Laura A Bass"
Article Synopsis
- A new method combines size exclusion chromatography (SEC) and mixed-mode liquid chromatography (LC) for analyzing various components in biopharmaceuticals.
- This approach can measure the active pharmaceutical ingredient (protein) along with different excipients like cations, anions, and sugars, using dual SEC columns for separation and detection through UV and evaporative light scattering detectors.
- The technique has been successfully applied to quantify proteins and excipients in various biopharmaceutical products, including monoclonal antibodies and vaccines, and allows for advanced online characterization with mass spectrometry.
A majority of the previous investigations on the hydrodynamic sensitivity of mammalian cells have focused on lethal effects as determined by cell death or lysis. In this study, we investigated the effect of hydrodynamic stress on CHO cells in a fed-batch process using a previously reported system which subjects cells to repetitive, high levels of hydrodynamic stress, quantified by energy dissipation rate (EDR). The results indicated that cell growth and monoclonal antibody production of the test cells were very resistant to the hydrodynamic stress.
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