Computerized restriction endonuclease analysis compared with O-serotype and phage type in the epidemiologic fingerprinting of Pseudomonas aeruginosa strains.

OBJECTIVE: To assess restriction endonuclease analysis (REA) of chromosomal DNA using SalI enzyme, low-concentration (0.4%) agarose gels and digitalized data management of the REA patterns obtained for the typing of clinical Pseudomonas aeruginosa isolates. METHODS: A group of 67 clinical unrelated isolates from 10 Spanish hospitals was used to study the discriminatory power, reproducibility and typeability of REA typing.

Prevalence of multiple sclerosis in the province of Teruel, Spain.

There have been few reports about the frequency of multiple sclerosis (MS) in Spain. We undertook a prevalence study in the province of Teruel, which is served by two hospitals as referral centres for a population of 143,680. We found a total of 46 patients who fulfilled Poser's criteria for clinically definite or probable MS with a prevalence rate of 32/100,000 [95% confidence interval (CI): 22.

[Stomach cancer mortality in Brazil: from 1977 to 1989].

Both incidence and mortality of stomach cancer have been declining in most of the world in the last decades. However, this phenomenon has not been studied in Brazil. Although data on incidence rates are insufficient, there is a standardized historical series on mortality data beginning in 1977.

[Health profile of children cared for in nurseries and the prevention of communication disorders].

For the purpose of characterizing the health of the population assisted by the Nursery Program (Programa Creche) at the three units financed by the city authorities of S. Paulo, Brazil, an enquiry was undertaken. A questionnaire consisting of both open and closed question concerning personal identification, personal antecedents, development and health was prepared and 133 of them were applied.

[An information system on live births and the use of its data in epidemiology and health statistics].

The Brazilian Ministry of Health implemented, in 1990, a System of Information of Live Births (SINASC) which introduced a Birth Certificate with a view to obtaining the total number of these events and their distribution according to epidemiological, demographic and clinical characteristics. It was decided to evaluate the System according to its coverage and the quality of information obtained, two years after its initial implementation. The population of this study consists of 15,142 hospital live births which occurred in five cities of the State of São Paulo, Brazil, in 1992.

[Lipid profile of metallurgic industry employees and its relationship with other risk factors].

Purpose: To study the lipid profile and its relation with other risk factors for coronary heart disease in a population of metallurgic workers in São Bernardo do Campo, SP.

Methods: In 1966 employees were determined: lipid profile after 12h fasting, height and weight and they answered a questionnaire about other risk factors. Diabetic and hypertensive were excluded, remaining 1586 cases, 1384 males, mean age 34.

[Diagnosis of anaerobic infection of the pleural fluid using gas-liquid chromatography].

Background: An assessment of the efficacy of the detection of volatile and non-volatile fatty acids that are produced by microorganisms aimed at the possible diagnosis of pleural empyema.

Methods: 106 pleural fluids were examined by gas-liquid chromatography in contrast to conventional aerobic and anaerobic cultures. The sample previously prepared (1 microliter) was injected in an Hewlett-Packard 5890 chromatograph, that was equipped with a fused silica capillary column and a flame ionization detector.

[Myocardial infarct and cerebral vascular disorders associated with high temperature and carbon monoxide in a metropolitan area of southeastern Brazil].

Many studies have been published about the relationship between life style and genetic risk factors and stroke and heart attack, but there have been few about the relationship between atmospheric pollution, specifically with carbon monoxide, and temperature and stroke and heart attack. With a view to filling this gap the relationship between values of carbon monoxide concentration in the air, maximum and average by day, and maximum temperature by day and the number of new cases of CVA and IM admitted as emergencies at the biggest hospital complex of S. Paulo city was analysed.

[Prevalence of obesity in a locality of the State of São Paulo, Brazil, 1987].

The prevalence of obesity in Araraquara, a county of 150,000 inhabitants situated 250 km from S. Paulo, Brazil was studied. The study population, of 18-74 yrs.

[Changes in ocular tone, pH, ascorbic acid and lactic acid concentration in the aqueous humor of rabbits treated with aceclidine].

The Authors state the results of their research on the modification of the aqueous humor caused by the instillation of the Clonidina to the concentrations of 0,I25% in a group of ten rabbits. The results of these studies illustrate the variations of pH, the ocular tone, the concentration of lactic and ascorbic acid. In all the rabbits examined Clonidina was instillated in the RE while in the LE distilled water was instillated for the control.

[Changes in ocular tonus, pH and concentration of ascorbic acid and lactic acid in the aqueous humor of rabbits treated with clonidine].

The authors state the results of their research on the modification of the aqueous humor caused by the instillation of the Clonidine in a group of ten rabbits. The results of these studies illustrate the variations of pH, the ocular tone, the concentration of lactic and ascorbic acid. In all the rabbits examined was instillated Clonidine for one hour in RE while in LE distilled water was instilled for the control.

[Changes in ocular tonus, pH and concentration of ascorbic acid and lactic acid in the aqueous humor of rabbits treated with epinephrine].

The authors state the results of their research on the modification of the aqueous humor caused by the instillation of the Epinephrine in a group of ten rabbits. The results of these studies illustrate the variations of pH, the ocular tone, the concentration of lactic and ascorbic acid. In all the rabbits examined Epinephrine was instillated in the RE while in the LE distilled water was instillated for the control.