IAA-producing bacteria from the rhizosphere of chickpea ( L.): Isolation, characterization, and their effects on plant growth performance.

Indole-3-acetic acid (IAA), a crucial plant hormone, regulates diverse physiological processes. This study aimed to isolate and characterize IAA-producing bacteria from the chickpea ( L.) rhizosphere and evaluate their effects on plant growth.

An Improved Helper Plasmid Containing Deletions Within the E4 and E2a Genes Results in Increased Adeno-Associated Virus Productivity.

The use of a helper plasmid to replace adenovirus infection for adeno-associated virus (AAV) manufacturing has been common practice for decades. Adenovirus E4, E2a, and VA RNA genes are sufficient to support efficient AAV replication. In an effort to ensure that all transfected DNA has a functional role in AAV production, deletions were introduced to the E4 and E2a genes to determine if any portions were dispensable.

CaC-induced ripening: Unveiling the bitter truth behind sweet fruit.

Fruit ripening is a natural, irreversible process crucial for developing luscious flavor and appealing appearance. Fruits are lauded for their health benefits, forming a key part of a balanced diet. Regrettably, the continued use of calcium carbide (CaC) to ripen fruit persists in various regions due to its low cost and perceived effectiveness.

Analytical characterization of full, intermediate, and empty AAV capsids.

Manufacturing of recombinant adeno-associated virus (AAV) vectors produces three types of capsids: full, intermediate, and empty. While there are different opinions about the impact of intermediate and empty capsids on safety and efficacy of AAV products, they are generally considered impurities because they are not the intended fully intact vector product. The presence of these impurities could impact product efficacy due to potential competition with fully packaged AAVs for cellular transduction, as well as have potential implications to patient safety due to increased capsid load during dosing.

Draft genomes of isolated from honeybee colonies () in Fiji.

Here, we report draft genomic sequences from three isolates, the causative agent of American Foulbrood (AFB), obtained from honeybee colonies of in Fiji, which allow both enterobacterial repetitive intergenic consensus and multilocus sequence typing genotypes to be elucidated for Fijian AFB.

Diagnostic Accuracy of Cerebrospinal Fluid (CSF) Adenosine Deaminase (ADA) for Tuberculous Meningitis (TBM) in Adults: A Systematic Review and Meta-Analysis.

Tuberculous meningitis is the most serious complication of tuberculosis. Early diagnosis is crucial to start relevant treatment to prevent death and disability. Electronic databases PubMed, Google Scholar, and Cochrane Library were used to find relevant articles from January 1980 to June 2022.

A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaption by a major maize pest insect.

Background: Adaptations by arthropod pests to host plant defenses of crops determine their impacts on agricultural production. The larval host range of western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), is restricted to maize and a few grasses. Resistance of D.

Genome size evolution in the beetle genus Diabrotica.

Diabrocite corn rootworms are one of the most economically significant pests of maize in the United States and Europe and an emerging model for insect-plant interactions. Genome sizes of several species in the genus Diabrotica were estimated using flow cytometry along with that of Acalymma vittatum as an outgroup. Genome sizes ranged between 1.

Effect of postharvest hydrogen sulphide on lignification and biochemical markers of pointed gourd.

Hydrogen sulphide (H S) has emerged as a gasotransmitter molecule that modulates several physiological functions in plants, specially, different biotic and abiotic stresses. Pointed gourd (PG) fruits start losing their soft texture and appealing colour within 2-3 days of harvest, resulting in poor marketability and shelf life. Here, the effect of exogenous H S (1 and 2 mm) application on lignification and other biochemical markers linked to ripening, shelf-life and edible quality of PG was assessed during cold (12 °C, 85-90% RH) and ambient (27 ± 2 °C, 55 ± 5% RH) storage.

Hydrogen sulphide infiltration downregulates oxidative metabolism and extends postharvest life of banana.

Hydrogen sulphide (H S) has emerged as a potential regulator of plant defence against different abiotic stresses. As a climacteric fruit, banana undergoes oxidative stresses shortly after harvest, resulting in faster ripening and senescence. This work examines the effects of vacuum infiltrated H S on ripening inhibition of banana.

The Duration of Intestinal Immunity After an Inactivated Poliovirus Vaccine Booster Dose in Children Immunized With Oral Vaccine: A Randomized Controlled Trial.

Background: In 2014, 2 studies showed that inactivated poliovirus vaccine (IPV) boosts intestinal immunity in children previously immunized with oral poliovirus vaccine (OPV). As a result, IPV was introduced in mass campaigns to help achieve polio eradication.

Methods: We conducted an open-label, randomized, controlled trial to assess the duration of the boost in intestinal immunity following a dose of IPV given to OPV-immunized children.

Comparison of Radicular Peroxide Leakage from four Commonly used Bleaching agents following Intracoronal Bleaching in Endodontically treated teeth - An In Vitro Study.

Background: Non vital bleaching is simple, conservative procedure for esthetic correction of discolored endodontically treated teeth. The aim of this study was to determine and compare the amount of peroxide leakage from four different bleaching agents i.e superoxol, sodium perborate, combination of superoxol & sodium perborate and carbamide peroxide during intracoronal bleaching, as the safe and effective bleaching is the need of the hour.

Influence of ultrasound, with and without water spray cooling, on removal of posts cemented with resin or glass ionomer cements: An in-vitro study.

Aims/objectives: To evaluate in vitro the ultrasonic vibration efficacy with and without water spray cooling on the reduction of the amount of force necessary to dislodge the cast posts cemented with resin cement and to compare it with those cemented with GIC Type I luting cement.

Materials And Methods: SIXTY SAMPLES WERE DIVIDED INTO SIX GROUPS: groups 1, 2, 3, posts cemented with GIC; groups 4, 5, 6, posts cemented with resin; groups 1 and 4 (controls), no ultrasound; groups 2 and 5, ultrasound without water spray; and groups 3 and 6, ultrasound with water spray. Instron testing machine was used to dislodge the posts from the root canals and the data was statistically analyzed.

An in-vitro SEM comparative study of debridement ability of K-Files and Canal Master.

The aim of this study was to compare in vitro the debridement ability of Canal Master and K-Files using scanning electron microscope. One hundred and twelve freshly extracted human upper central incisor teeth were divided into two groups of 56 each. One group was instrumented using Canal Master and another group was instrumented using K-Files.