Publications by authors named "Lars Wommer"
Article Synopsis
- - The study developed an automated long-term fermentation process for fungi grown in pellet form to prevent problems like overgrowth in bioreactors and improve key properties of the fermentation broth, such as oxygen transfer and mixing time.
- - Instead of using continuous culture or fed-batch fermentation, a repeated-batch fermentation method was employed with special bioreactor internals called "sporulation supports" to capture and retain biomass during fermentation.
- - After harvesting the biomass, an intermediate cleaning was conducted, and the retained pellets underwent a sporulation phase to produce spores, which were then used to inoculate the next batch, ensuring consistent spore concentration and avoiding issues from undesirable fungal growth.
Laboratory protocols using magnetic beads have gained importance in the purification of mRNA for vaccines. Here, the produced mRNA hybridizes specifically to oligo(dT)-functionalized magnetic beads after cell lysis. The mRNA-loaded magnetic beads can be selectively separated using a magnet.
View Article and Find Full Text PDFPurification of mRNA with oligo(dT)-functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can result in a decreased total surface area and thus a decreased yield of mRNA. In addition, agglomeration may also be caused by mRNA loading on the magnetic particles.
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