Publications by authors named "Larry T Clayton"
Background: Iohexol is an iodinated contrast dye that has been shown to be useful in the estimation of glomerular filtration rate (GFR) in patients with suspected renal insufficiency. We developed and validated an ultraperformance liquid chromatography (UPLC)-triple quadrupole mass spectrometry (MS/MS) assay for quantifying iohexol in human serum.
Methods: Sample preparation involved dilution of 50 microL serum with 400 microL water, followed by protein precipitation with zinc sulfate and methanol containing the structural analog ioversol as the internal standard.
Background: The potent immunosuppressant mycophenolic acid (MPA) is metabolized to an inactive glucuronide (MPAG). The extent of metabolism varies among individuals, and the MPAG formed can be hydrolyzed to MPA and can displace MPA from serum albumin, creating a potential need to monitor both MPA and MPAG.
Methods: After addition of the carboxybutoxy ether of MPA (MPAC) as internal standard, MPA and MPAG were isolated from serum by acidification followed by solid-phase extraction.