Two-stage system for hydrogen production by immobilized cyanobacterium Gloeocapsa alpicola CALU 743.

Previous studies showed that cell suspensions of unicellular nondiazotrophic cyanobacterium G. alpicola grown under nitrate-limiting conditions intensively produces H2 via fermentation of endogenous glycogen with hydrogen yield more then 90% of theoretical maximum (3.8 mol H2 per mol glucose).

Identification of hox genes and analysis of their transcription in the unicellular cyanobacterium Gloeocapsa alpicola CALU 743 growing under nitrate-limiting conditions.

The unicellular non-N(2)-fixing cyanobacterium Gloeocapsa alpicola CALU 743 contains a bidirectional hydrogenase. Parts of all structural genes, encoding the hydrogenase, were identified, cloned and sequenced. When comparing the sequences with analogous sequences from other cyanobacteria the highest similarity was observed with hox genes from Synechocystis sp.