Bioprospecting by Phage Display of Mimetic Peptides of for Use in Laboratory Diagnosis.

Background: infection is a major public health problem and the most common sexually transmitted infection in the world. Although highly prevalent, 70% to 80% of cases are asymptomatic and undiagnosed.

Purpose: To overcome some limitations in terms of rapid diagnosis, phage display technology was used to bioprospect peptide mimetics of immunoreactive and immunogenic antigens to be selected for the production of synthetic peptides.

In situ detection of , and cytokines among cardiovascular diseased patients from the Amazon region of Brazil.

Background: Chronic coronary artery disease has been associated, as a consequence of the local inflammatory reaction with previous or persistent infection with , which led to the investigation of the association of cardiovascular disease and previous infection with and the role of cytokine profile (in situ) markers in the vascular system tissues.

Methods: Sixty-nine biopsies were collected for immunohistochemical analysis for the presence of IL-6, IL-8, TNF-α, IFN-γ, TGF-β, and IL-10, in 16 fragments from atheromatous plaques, 32 aorta fragments, and 21 valve fragments, using a tissue microarray technique for paraffin embedded tissues.

Results: Most patients undergoing revascularization surgery were men >50 years, while those undergoing valve replacement were mostly women <50 years.