Novel Proteins of the High-Affinity Nitrate Transporter Family NRT2, SaNRT2.1 and SaNRT2.5, from the Euhalophyte : Molecular Cloning and Expression Analysis.

Two genes of nitrate transporters and , putative orthologs of high-affinity nitrate transporter genes and from , were cloned from the euhalophyte . Phylogenetic bioinformatic analysis demonstrated that the proteins SaNRT2.1 and SaNRT2.

Molecular Cloning, Expression and Transport Activity of SaNPF6.3/SaNRT1.1, a Novel Protein of the Low-Affinity Nitrate Transporter Family from the Euhalophyte (L.) Pall.

The gene, a putative ortholog of the dual-affinity nitrate (NO) transporter gene / from , was cloned from the euhalophyte . The nitrate transporting activity of SaNPF6.3 was studied by heterologous expression of the gene in the yeast () mutant strain Δ lacking the original nitrate transporter.

Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.

Researchers are often interested in proteins that are present in cells in small ratios compared to the total amount of proteins. These proteins include transcription factors, hormones and specific membrane proteins. However, sufficient amounts of well-purified protein preparations are required for functional and structural studies of these proteins, including the creation of artificial proteoliposomes and the growth of protein 2D and 3D crystals.

Chloride Channel Family in the Euhalophyte (L.) Pall: Cloning of Novel Members SaCLCa2 and SaCLCc2, General Characterization of the Family.

CLC family genes, comprising anion channels and anion/H+ antiporters, are widely represented in nearly all prokaryotes and eukaryotes. CLC proteins carry out a plethora of functions at the cellular level. Here the coding sequences of the SaCLCa2 and SaCLCc2 genes, homologous to Arabidopsis thaliana CLCa and CLCc, were cloned from the euhalophyte Suaeda altissima (L.

Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the Halophyte (L.) Pall.

Coding sequences of the CLC family genes , , and , the putative orthologs of , , and genes, were cloned from the euhalophyte (L.) Pall. The key conserved motifs and glutamates inherent in proteins of the CLC family were identified in SaCLCd, SaCLCf, and SaCLCg amino acid sequences.

Cloning and Characterization of Two Putative P-Type ATPases from the Marine Microalga Similar to Plant H-ATPases and Their Gene Expression Analysis under Conditions of Hyperosmotic Salt Shock.

The green microalga genus is mostly comprised of species that exhibit a wide range of salinity tolerance, including inhabitants of hyperhaline reservoirs. Na content in cells inhabiting saline environments is maintained at a fairly low level, comparable to that in the cells of freshwater organisms. However, despite a long history of studying the physiological and molecular mechanisms that ensure the ability of halotolerant species to survive at high concentrations of NaCl, the question of how cells remove excess Na ions entering from the environment is still debatable.

Functional identification of electrogenic Na+-translocating ATPase in the plasma membrane of the halotolerant microalga Dunaliella maritima.

The hypothesis that the primary Na+-pump, Na+-ATPase, functions in the plasma membrane (PM) of halotolerant microalga Dunaliella maritima was tested using membrane preparations from this organism enriched with the PM vesicles. The pH profile of ATP hydrolysis catalyzed by the PM fractions exhibited a broad optimum between pH 6 and 9. Hydrolysis in the alkaline range was specifically stimulated by Na+ ions.

The Na+-translocating ATPase in the plasma membrane of the marine microalga Tetraselmis viridis catalyzes Na+/H+ exchange.

Our previous investigations have established that Na+ translocation across the Tetraselmis viridis plasma membrane (PM) mediated by the primary ATP-driven Na+-pump, Na+-ATPase, is accompanied by H+ counter-transport [Y.V. Balnokin et al.