Publications by authors named "Larise Oberholster"
Article Synopsis
- Up to 46% of patients with suspected autoimmune limbic encephalitis don’t test positive for known central nervous system antibodies, prompting the development of a new cell-based assay (CBA) for detecting novel neural antibodies using human-induced pluripotent stem cells (hiPSCs).
- The study involved testing serum and cerebrospinal fluid from 99 patients with inflammatory and non-inflammatory neurological diseases to identify IgG binding to hiPSC-derived neurons and astrocytes using advanced fluorescence techniques.
- The CBA successfully detected neural-specific antibodies in 19 out of 99 patients, with a higher prevalence in those with inflammatory neurological diseases compared to non-inflammatory cases, underscoring its potential in identifying previously unknown autoantibodies.
Extracellular vesicles (EVs) are membrane-bound vesicles secreted by all cell types that play a central role in cell-to-cell communication. Since these vesicles serve as vehicles of cellular content (nucleic acids, proteins and lipids) with the potential to cross biological barriers, they represent a novel attractive window into an otherwise inaccessible organ, such as the brain. The composition of EVs is cell-type specific and mirrors the physiological condition of the cell-of-origin.
View Article and Find Full Text PDFProgressive multifocal leukoencephalopathy is a crimpling demyelinating disease of the central nervous system caused by JC polyomavirus (JCPyV). Much about JCPyV propagation in the brain remains obscure because of a lack of proper animal models to study the virus in the context of the disease, thus hampering efforts toward the development of new antiviral strategies. Here, having established a robust and representative model of JCPyV infection in human-induced pluripotent stem cell-derived astrocytes, we are able to fully characterize the effect of JCPyV on the biology of the cells and show that the proteomic signature observed for JCPyV-infected astrocytes is extended to extracellular vesicles (EVs).
View Article and Find Full Text PDFBackground And Objective: Depleting CD20 B cells is the primary mechanism by which ocrelizumab (OCRE) is efficient in persons with multiple sclerosis (pwMS). However, the exact role of OCRE on other immune cell subsets directly or indirectly remains elusive. The purpose of this study is to characterize the dynamics of peripheral immune cells of pwMS on OCRE.
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