Application of the antibiotic batumin for accurate and rapid identification of staphylococcal small colony variants.

Background: Staphylococcus aureus is a major human pathogen causing significant morbidity and mortality. The S. aureus colonies in osteomyelitis, in patients with cystic fibrosis and patients with endoprosthesis rejection frequently have an atypical morphology, i.

Antibiotic batumin for diagnostics of staphylococci and treatment of Staphylococcus aureus nasal carriage.

Paper disks containing 5 microg of antibiotic batumin (preparation "Diastaph") were tested using standard disk diffusion technique against 1520 strains of bacteria (clinical isolates of different genera and type strains of staphylococci and micrococci). Presence of growth inhibition zone 17 mm and more around the disk gave evidence that the isolate belonged to the genus Staphylococcus. Method ensured quick and reliable identification of all strains of this genus.

Phenotypic peculiarities of batumin-resistant staphylococci.

Batumin-resistant variants of two clinical strains of Staphylococcus (S.) aureus and of strain ATCC 6538 P (209 P) were produced in vitro by continuous subculture in the presence of batumin. Their phenotypic characteristics differed substantially from the parent strains properties and did not correspond to those of S.