Publications by authors named "Lara Sweetapple"
RNA regulation can be performed by a second targeting RNA molecule, such as in the microRNA regulation mechanism. Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) probes the structure of RNA molecules and can resolve RNA:protein interactions, but RNA:RNA interactions have not yet been addressed with this technique. Here, we apply SHAPE to investigate RNA-mediated binding processes in RNA:RNA and RNA:RNA-RBP complexes.
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- RNA-binding proteins (RBPs) have a modular structure due to multiple RNA-binding domains (RBDs), which help them interact specifically with RNA.
- This study focuses on the Unr protein to explore how these multiple RBDs work together to bind to single-stranded RNA (ssRNA), revealing that some RBDs do not bind RNA but help maintain the protein's structure.
- The findings suggest that interactions between active and inactive RBDs shape RNA recognition and binding, impacting processes like translation regulation and the overall RNA interactome.
Topological distributions of individual cellular clocks have not been demonstrated in peripheral organs. The cochlea displays circadian patterns of core clock gene expression [1, 2]. PER2 protein is expressed in the hair cells and spiral ganglion neurons of the cochlea in the spiral ganglion neurons [1].
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