Clock and riboswitch control of in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis.

Metabolic homeostasis is regulated by enzyme activities, but the importance of regulating their corresponding coenzyme levels is unexplored. The organic coenzyme thiamine diphosphate (TDP) is suggested to be supplied as needed and controlled by a riboswitch-sensing mechanism in plants through the circadian-regulated gene. Riboswitch disruption negatively impacts plant fitness.