Reading Primary Scientific Literature: Approaches for Teaching Students in the Undergraduate STEM Classroom.

Teaching undergraduate students to read primary scientific literature (PSL) is cited as an important goal for many science, technology, engineering, and math (STEM) classes, given a range of cognitive and affective benefits for students who read PSL. Consequently, there are a number of approaches and curricular interventions published in the STEM education literature on how to teach students to read PSL. These approaches vary widely in their instructional methods, target student demographic, required class time, and level of assessment demonstrating the method's efficacy.

Identification of Bacterial Taxa Present in a Concrete Pennsylvania Bridge.

Concrete contains low microbial biomass, but some bacteria can grow in this highly alkaline environment. We used silica-based DNA extraction and 16S rRNA sequence analysis to identify the bacteria in a corroded concrete bridge sample from Bethlehem, Pennsylvania. Staphylococcus, Streptococcus, , , , and were the most abundant genera.

A case study on genomic imprinting facilitates student learning.

Problem-based learning encourages students to deepen their understanding of a concept by working through a real-world example of course content. Case studies represent a form of problem-based learning that engages students in realistic scenarios to achieve a deeper understanding of concepts. Case studies have been shown to facilitate the learning of challenging subject matter.

Professional science education videos improve student performance in nonmajor and intermediate biology laboratory courses.

Undergraduate biology laboratories emphasize hands-on skills. Typically, descriptions of manual techniques are delivered via written instruction. Custom-produced prelab instructional videos, which augment prelab instruction, have come into wider use in recent years.

Integrating cell and molecular biology concepts: Comparing learning gains and self-efficacy in corresponding live and virtual undergraduate laboratory experiences.

Multiple pedagogical approaches, such as experimental experiences or computer-based activities, have been shown to increase student learning and engagement. We have developed a laboratory module that includes both a traditional "live" experimental component and a student-designed "virtual" computer simulation component. This laboratory employs the mating pathway of Saccharomyces cerevisiae (yeast) to demonstrate four fundamental cell and molecular biology concepts: cell signaling, cytoskeleton, cell cycle, and cell cycle checkpoints.

Stressing Escherichia coli to educate students about research: A CURE to investigate multiple levels of gene regulation.

Course-based undergraduate research experiences (CUREs) have been shown to increase student retention and learning in the biological sciences. Most CURES cover only one aspect of gene regulation, such as transcriptional control. Here we present a new inquiry-based lab that engages understanding of gene expression from multiple perspectives.

S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association.

In diverse organisms, the Mre11 complex and phosphoinositide 3-kinase-related kinases (PIKKs), such as Tel1p and Mec1p from S. cerevisiae, are key mediators of DNA repair and DNA damage checkpoints that also function at telomeres. Here, we use chromatin immunoprecipitation (ChIP) to determine if Mre11p, Tel1p, or Mec1p affects telomere maintenance by promoting recruitment of telomerase subunits to S.

The Saccharomyces cerevisiae helicase Rrm3p facilitates replication past nonhistone protein-DNA complexes.

The Saccharomyces cerevisiae RRM3 gene encodes a 5' to 3' DNA helicase. While replication of most of the yeast genome was not dependent upon Rrm3p, in its absence, replication forks paused and often broke at an estimated 1400 discrete sites, including tRNA genes, centromeres, inactive replication origins, and transcriptional silencers. These replication defects were associated with activation of the intra-S phase checkpoint.