Hepatic lesions in Lebistes reticulatus induced by N-nitrosodiethylamine.

The effect of N-nitrosodiethylamine (NDEA) on the aquarium fish, Lebistes reticulatus (guppy) has been investigated. The aquarium water contained 10 to 200 parts per million of NDEA. The LC50/30 for NDEA was 100 ppm.

epsilon-N-trimethyl-lysine: a natural cell component with mitogenic activity.

The blastogenic capacity of a new mitogenic agent, epsilon-N-trimethyl-lysine (TML) was examined on human peripheral blood lymphocytes. The changes in relative DNA and histone content after TML treatment testified that activated lymphocytes passed through the whole cell cycle. The comparison of the 3H-TdR incorporation rate into unseparated T-B lymphocyte and purified T lymphocyte cultures suggested a preferential effect on the thymidine incorporation of T lymphocytes.

[Determination of the copper content of liver tissue from paraffin-imbedded samples by electron spin resonance spectroscopy].

The Electron Spin Resonance spectrum of human liver specimens embedded in paraffin was investigated. An easily registrable mark could be seen in the g 2.05 range of the spectrum.

Morphological and biochemical study of the hepatoprotective effect of AICA-phosphate.

The hepatoprotective effect and the possible mechanism of action of AICA-phosphate (AIP) were studied. Both the morphological and biochemical data proved the protective i.e.

Acridine orange staining of the mammalian fibroblast cell coat.

Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compounds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours.

[Scanning electron microscopy of oral leukoplakia].

Buccal mucosa of six patients with leukoplakia and normal controls was examined by scanning electron microscopy. Findings have shown that: 1. there is a difference between the surface epithelial cells of the normal mucosa and those of the mucosa with leukoplakia; 2.

[Electron microscopic changes in the liver in systemic lupus erythematosus].

Liver biopsy specimen of a patient with systemic lupus erythematosus was studied by light- and electron microscopy. On the background of the nonspecific hepatitis of a mild degree ultrastructural changes of the liver were revealed. Diffuse damage to the mitochondria and the vascular pole of the cells have been found.

Chromatin proteins as a possible target for antitumour agents: alterations of chromatin proteins in dibromodulcitol treated Yoshida tumors.

The effect of dibromodulcitol (DBD) on Yoshida sarcoma chromatin components has been investigated. Measurements on the radioactivity of nuclear components after in vivo treatment with [3H]DBD for 1 h indicated preferential drug binding to the high molecular weight component of the nuclear residual acidic protein (non-histones) and also to Histone 1 (H1) (very lysine rich, F1). Two-hour DBD treatment resulted in partial degradation and reduced [3H]leucine incorporation into the same fractions of chromatin.

[Myasthenia gravis: epithelial cells of the hyperplastic thymus. Morphometric and ultrastructural study].

Epithelial cells of hyperplastic thymuses, removed because of myasthenia gravis were studied electronmicroscopically and morphometrically. The correlation between the age of patients and the number of epithelial cells of the thymus is discussed. Various type of epithelial cells are distinguished.

[Ultrastructure and classification of epithelial thymomas].

4 epithelial thymomas out of 36 various types of it were studied electronmicroscopically. The following types were distinguished: clear cell thymoma, dark cell, solid thymoma, lacunar and spindle cell thymoma. These types of thymomas differ not only morphologically, but clinically.

Electron spin resonance (ESR) spectrum of paraffin embedded human liver tissue. A possible method for the estimation of copper content.

Human liver tissues embedded in paraffin wax for histological examination have been studied by Electron Spin Resonance (ESR) spectroscopy. A signal was detected at g approximately 2.05 section of the spectrum.

Comparative study of tumor-specific transplantation antigens of MC-29 chicken hepatoma and Rous sarcoma virus-induced sarcomas in mice.

Immunization of CBAT6T6 mice with MC-29 hepatoma antigen did not change the take of Rous sarcoma virus, Schmidt-Ruppin strain [RSV(SR)] mouse tumors after sc transplantation. Immunization with MC-29 hepatoma antigen only slightly increased the average survival time of the mice and significantly decreased tumor growth only at the minimal lethal dose level. Immunization of mice with MC-29 hepatoma antigen and immunization with chicken Rous sarcoma gave similar results; both elicited much less transplantation resistance than immunization with irradiated RSV(SR) mouse tumor cells.

Chromatin alterations and gene function disorder in MC-29 virus-derived hepatoma.

The disorder of gene expression in hepatomas was studied by following certain metabolic alterations (enzyme stimulation, nucleic acid labeling) after glucocorticoid treatment and analyzing the site of action of glucocorticoids. Compared to normal liver, the MC-29 virus-derived transplantable hepatoma (VTH) responded abnormally to glucocorticoids, which failed to stimulate the activity of certain enzymes (glucose-6-phosphatase, aryl hydrocarbon hydroxylase) or to inhibit DNA synthesis. Since the binding capacity of the cytosol steroid receptor was the same in liver and VTH but the interaction between the steroid receptor and DNA was reduced in VTH, it was concluded that structural alterations of chromatin nonhistones--including processed steroid receptor--may be responsible for the lack of physiological responses to steroids in VTH.

Biochemical behavior of MC-29 virus-induced transplantable chicken hepatoma.

Studies were performed to test the applicability of the molecular correlation concept developed in chemically induced transplantable rat hepatomas to virally induced transplantable hepatoma in chicken. In a comparison of the activities of carbohydrate, purine, and pyrimidine key enzymes in rat and chicken hepatomas, similar patterns were observed in rodent and avian tumors. These results show that the enzymatic imbalance elucidated in chemically induced rat hepatomas is applicable to the virus-induced hepatoma in chicken, independent of the nature of the carcinogenic agent and the species.

Histology and ultrastructural aspects of virus-induced primary liver cancer and transplantable hepatomas of viral origin in chickens.

The macroscopic, light microscopic and electron microscopic features and biological properties of MC-29 virus-induced hepatocellular carcinomas in chickens are described. The tumors developed in noncirrhotic livers within a very short time and formed metastases. Virus production was also evidenced in the tumors.

Pathology of primary liver cancer.

After a brief survey of the factors that play a role in the etiopathogenesis of human hepatocellular carcinomas, a detailed description is given of the macroscopic and microscopic features of human liver cancers as well as their association with cirrhosis. The ultrastructural features of liver cancers of various degrees of differentiation are described. The mode of spread, metastasis formation of primary liver cancers, and most frequent causes of death of liver cancer patients are reviewed.

Structure of functioning saphenous venous grafts.

The changes developing in functioning saphenous vein transplants were studied by light and electron microscopy in three human cases. The degenerative signs appearing in the smooth muscle cells of the graft, as well as the glycogen accumulation and the changes of the cell organelles are discussed. Beside the thick elastic fibres visible under the light microscope in the graft functioning for 22 months the electron microscope revealed as a characteristic lesion the development of elastic fibres among the young collagenous fibres.