Aptamer's Structure Optimization for Better Diagnosis and Treatment of Glial Tumors.

Oncological diseases are a major focus in medicine, with millions diagnosed each year, leading researchers to seek new diagnostic and treatment methods. One promising avenue is the development of targeted therapies and rapid diagnostic tests using recognition molecules. The pharmaceutical industry is increasingly exploring nucleic acid-based therapeutics.

Visualization of Brain Tumors with Infrared-Labeled Aptamers for Fluorescence-Guided Surgery.

Gliomas remain challenging brain tumors to treat due to their infiltrative nature. Accurately identifying tumor boundaries during surgery is crucial for successful resection. This study introduces an innovative intraoperative visualization method utilizing surgical fluorescence microscopy to precisely locate tumor cell dissemination.

[Long-term results of ureteral replacement using small bowel in patients with long strictures: 9-years single-center experience].

Introduction: When reconstructing long ureteral strictures, the optimal substitution material is reconfigured pelvis or bladder flaps. However, it is not always possible to use them due to involvement in the pathological process or insufficient length to replace the defect. In such cases, substitution of the ureter by ileal segment is successfully used.

Monitoring of breast cancer progression via aptamer-based detection of circulating tumor cells in clinical blood samples.

Breast cancer (BC) diagnostics lack noninvasive methods and procedures for screening and monitoring disease dynamics. Admitted CellSearch is used for fluid biopsy and capture of circulating tumor cells of only epithelial origin. Here we describe an RNA aptamer (MDA231) for detecting BC cells in clinical samples, including blood.

Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors.

One of the promising novel methods for radical tumor resection at a single-cell level is magneto-mechanical microsurgery (MMM) with magnetic nano- or microdisks modified with cancer-recognizing molecules. A low-frequency alternating magnetic field (AMF) remotely drives and controls the procedure. Here, we present characterization and application of magnetic nanodisks (MNDs) as a surgical instrument ("smart nanoscalpel") at a single-cell level.

Development of DNA aptamers for visualization of glial brain tumors and detection of circulating tumor cells.

Here, we present DNA aptamers capable of specific binding to glial tumor cells , , and for visualization diagnostics of central nervous system tumors. We selected the aptamers binding specifically to the postoperative human glial primary tumors and not to the healthy brain cells and meningioma, using a modified process of systematic evolution of ligands by exponential enrichment to cells; sequenced and analyzed ssDNA pools using bioinformatic tools and identified the best aptamers by their binding abilities; determined three-dimensional structures of lead aptamers (Gli-55 and Gli-233) with small-angle X-ray scattering and molecular modeling; isolated and identified molecular target proteins of the aptamers by mass spectrometry; the potential binding sites of Gli-233 to the target protein and the role of post-translational modifications were verified by molecular dynamics simulations. The anti-glioma aptamers Gli-233 and Gli-55 were used to detect circulating tumor cells in liquid biopsies.

Laser Cleaning Improves Stem Cell Adhesion on the Dental Implant Surface during Peri-Implantitis Treatment.

Dental implant therapy is a well-accepted treatment modality. Despite good predictability and success in the early stages, the risk of postplacement inflammation in the long-term periods remains an urgent problem. Surgical access and decontamination with chemical and mechanical methods are more effective than antibiotic therapy.

Synthesis and Growth of Rare Earth Borates NaSrR(BO) (R = Ho-Lu, Y, Sc).

NaSrR(BO) (R = Ho-Lu, Y, Sc) compounds were obtained for the first time. Their structures exhibit disordered positions of Sr and Na atoms while RO polyhedra are connected through the BO groups. Large distances between R atoms and high transparency in the range of 250-900 nm make them promising for phosphor applications.

Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood.

We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability.

[Electroencephalographic markers of alexithymia in patients with moderate depression].

Objective: To search for electroencephalographic markers of alexithymia in patients with moderate depression.

Material And Methods: Sixty-four right-handed inpatients (20 men and 44 women, mean age 29.3+10.

Aptamer-Conjugated Superparamagnetic Ferroarabinogalactan Nanoparticles for Targeted Magnetodynamic Therapy of Cancer.

Nanotechnologies involving physical methods of tumor destruction using functional oligonucleotides are promising for targeted cancer therapy. Our study presents magnetodynamic therapy for selective elimination of tumor cells in vivo using DNA aptamer-functionalized magnetic nanoparticles exposed to a low frequency alternating magnetic field. We developed an enhanced targeting approach of cancer cells with aptamers and arabinogalactan.

The Discovery of RNA Aptamers that Selectively Bind Glioblastoma Stem Cells.

Glioblastoma (GBM) is the most aggressive primary brain tumor in adults. Despite progress in surgical and medical neuro-oncology, prognosis for GBM patients remains dismal, with a median survival of only 14-15 months. The modest benefit of conventional therapies is due to the presence of GBM stem cells (GSCs) that cause tumor relapse and chemoresistance and, therefore, that play a key role in GBM aggressiveness and recurrence.

Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood.

We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies.

Comparative Study of Physicochemical and Antibacterial Properties of ZnO Nanoparticles Prepared by Laser Ablation of Zn Target in Water and Air.

Here, we report on ZnO nanoparticles (NPs) generated by nanosecond pulsed laser (Nd:YAG, 1064 nm) through ablation of metallic Zn target in water and air and their comparative analysis as potential nanomaterials for biomedical applications. The prepared nanomaterials were carefully characterized in terms of their structure, composition, morphology and defects. It was found that in addition to the main wurtzite ZnO phase, which is conventionally prepared and reported by others, the sample laser generated in air also contained some amount of monoclinic zinc hydroxynitrate.

Copper Nanoparticles for Ascorbic Acid Sensing in Water on Carbon Screen-printed Electrodes.

A carbon screen-printed electrode was modified with copper nanoparticles from their dispersion obtained via pulsed laser ablation of a copper target in ethanol. The modified electrode exhibited activity in ascorbic acid electrochemical oxidation, giving an anodic current peak on voltammograms. Linear sweep and cyclic voltammetry have shown a linear dependence of the signal (both peak height and peak area) on the ascorbic acid concentration.

Ferroelectric polymer scaffolds based on a copolymer of tetrafluoroethylene with vinylidene fluoride: fabrication and properties.

A solution blow spinning technique is a method developed recently for making nonwoven webs of micro- and nanofibres. The principal advantage of this method compared to a more traditional electrospinning process is its significantly higher production rate. In this work, the solution blow spinning method was further developed to produce nonwoven polymeric scaffolds based on a copolymer of tetrafluoroethylene with vinylidene fluoride solution in acetone.

[Diurnal changes of alpha-rhythm in endogenous depressions].

The aim of the study was to find the diurnal changes in the EEG alpha-activity in patients with endogenous depressions. Both morning and evening EEG records were characterized by the transition of modal value of leading rhythm into the lower frequency band (9-10 Hz versus 10-11 Hz in healthy people). The spectral power of alpha-rhythm was higher, especially in the occipital cortical areas, in the morning hours than in the evening.

[Children's health protection in the state political system].

The system of children's health protection in this country still lacks an adequate legal basis. Its improvement should be considered as a function of the state realized through legal regulation of public relations for the benefit of each child based on the legislatively fixed government policy in the sphere of children's health care. Such an approach may strengthen the role of this sphere in the intersectoral relations and implies the extension of pediatrics toward interaction with the spheres of politics and law.

[Pantogam in the treatment of patients with organic brain lesions of different genesis].

We studied 35 patients with organic brain lesions caused by secondary brain harmful factors (group 1) and 33 patients with organic brain diseases of vascular genesis (group 2). Mean age of patients was 45.5±2.

[Changes of EEG coherence in different stages of the formation of the psychoorganic syndrome].

The group of liquidators of Chernobyl Nuclear Power Plant accident (n=170) with nonpsychotic mental disorders of exogenic-organic origin was studied. Patients were stratified into five groups on the basis of dominating symptoms in different stages of disorders: group 1 included patients with the prevalence of asthenic symptoms (n=104); group 2 - with the prevalence of autonomous disorders (n=37); group 3 - with the marked neurotic-like symptom complex (n=10); group 4 - with depressive symptoms (n=10); group 5 - with disturbances of cognitive function. The control group included 150 healthy people.

Neurokynurenines (NEKY) as common neurochemical links of stress and anxiety.

The following NEKY have been studied: 1-kynurenine (KYN), 3-hydroxyKYN (3HKYN), kynurenic (KYNA), anthranilic (ANT), 3-hydroxyANT (3HANT), quinolinic (QUIN), picolinic (PICA), xanthurenic (XAN), nicotinic (NIC) acids, 3-indole-pyruvate (IPA), nicotinamide (NAM). NEKY antagonize the central effects of precursors of serotonin (tryptophan and 5-HTP), and tryptamine as well. Seizures induced by central administration of KYN and QUIN are prevented by centrally injected dopamine and diminished by noradrenaline and adrenaline.

Phenibut (beta-phenyl-GABA): a tranquilizer and nootropic drug.

Phenibut (beta-phenyl-gamma-aminobutyric acid HCl) is a neuropsychotropic drug that was discovered and introduced into clinical practice in Russia in the 1960s. It has anxiolytic and nootropic (cognition enhancing) effects. It acts as a GABA-mimetic, primarily at GABA(B) and, to some extent, at GABA(A) receptors.