[The immunobiological properties of a corpuscular pertussis vaccine inactivated by gamma irradiation].

As a result of the high-dose gamma-irradiation (50 kGy) of corpuscular pertussis vaccine (CPV), pertussis radiovaccine (PRV) was obtained. Compared with CPV, PRV was shown to possess reduced toxicity and higher protective potency. Its adjuvant properties remained at the initial level, while its leukocytosis-promoting and histamine-sensitizing activities drastically decreased.

[The standardization of the in-vitro tetanus toxin neutralization test on Chinese hamster ovary cells].

A test for the titration of B. pertussis toxin with antisera on Chinese hamster ovary (CHO) cells has been worked out. B.

[The phenotypic properties of freshly isolated strains of Bordetella].

As the result of our investigations, newly isolated B. pertussis and B. bronchiseptica strains were studied.

[Structural organization of a segment of chromosome, containing the vir gene of Bordetella pertussis].

To study the structural arrangement of the chromosomal region containing vir genes of Bordetella pertussis the corresponding 15 kb fragment of Bordetella pertussis chromosomal DNA has been cloned. The sequence homology to an earlier characterized Bordetella pertussis genetical element RSBP1 and flanked by two 400 bp inverted repeats has been shown to be located at an end of a BamHI fragment. The restriction map of Bordetella pertussis 475 coincides with the previously published maps of Bordetella pertussis Tohama and 18323 permitting one to conclude the definite conservatism of the cloned sequence.

[Mobile element RSBP1 of Bordetella pertussis].

A number of repeated sequences was identified in the chromosome of Bordetella pertussis by the electron microscopic analysis of the chromosomal DNA of this microorganism. One of the sequences was cloned on the vector plasmid pHC79. It is shown to consist of two elements RSBP1 and RSBP2.

[The formation of a test system for assessing the safety of different types of pertussis preparations: their cytotoxic action on mouse thymocytes in vitro].

The test for the evaluation of the toxicity of different types of pertussis preparations as manifested by their in vitro influence on mouse thymic cells (T test) has been finally worked out. The use of the T test has made it possible to reveal the nonstandard character of the production lots of adsorbed diphtheria-pertussis-tetanus vaccines, both whole-cell vaccine and Japanese acellular vaccine. The degree of the in vitro damaging action of pertussis preparations on mouse thymic cells greatly depends on the residual content of Bordetella pertussis nontoxoidized toxin which, in contrast to B.

[The effect of gram-negative bacteria lipopolysaccharides on the development of Rauscher leukosis in BALB/c mice].

The dose-dependent action of Shigella sonnei lipopolysaccharide (LPS) on the development of acute erythroleukocytosis, as well as Rauscher chronic myeloid and lymphoid leukosis, in BALB/c mice sensitive to Rauscher virus was shown. Bordetella pertussis LPS in the doses used in this investigation stimulated the development of both acute erythroleukosis and chronic myeloid and lymphoid leukosis in BALB/c mice infected with Rauscher virus. Lipid A isolated from B.

[Evaluation of postvaccinal pertussis immunity by using immunoenzyme analysis].

The effectiveness of adsorbed DPT vaccine manufactured in the USSR, evaluated by its capacity of inducing the formation of the main classes of immunoglobulins and by the duration of immune response to the acellular complex of protective antigens (pertussis toxin and agglutinogen-2), was studied with the use of modified EIA. Out of 273 children immunized with adsorbed DPT vaccine in the course of this study, 87.2% had IgG-antibodies, 14.

[Polymyxin B suppresses the stimulating action of pertussis vaccine on hematopoiesis in mice].

The injection of killed whole-cell pertussis vaccine (PV), prepared from formulated Bordetella pertussis strains 5574 and 305, into mice was shown to produce a stimulating effect on hematopoiesis. This effect was manifested by an increase in the number of endogenic colonies developing in the spleen of mice on days 5 and 9 after their irradiation in a sublethal dose, by the sharp stimulation of the proliferative activity of splenic colony-forming units (CFUs) originating in the bone marrow and by the elevated CFUs level in the peripheral blood. The preliminary incubation of whole-cell PV with polymyxin B, cationic polypeptide selectively reacting with the lipid A moiety of lipopolysaccharide (LPS), led to a sharp decrease in the above-mentioned manifestations of hematopoietic effect of the vaccine, while incubation with cetavlon interacting with the polysaccharide moiety of LPS produced practically no effect on the capacity of the vaccine for stimulating hematopoiesis.

All species of the genus Bordetella contain genes for pertussis toxin of Bordetella pertussis.

A molecular probe for the PT-operon of B. pertussis hybridized with 4.7 Kb EcoRI-fragments of chromosomal DNAs of B.

Bacteriophages of Bordetella sp.: features of lysogeny and conversion.

It has been the purpose of this paper to study molecular-biological features of the Bordetella bacteriophage interaction with the host cell during lysogeny and conversion as well as to determine the degree of homology between genomes of homologous and heterologous bacteriophages. Genomes of bacteriophages from B. pertussis 134, 41405 and B.

[Antigen-nonspecific suppression of the immune response in mice as affected by pertussis vaccine].

A study was made of the suppressorgenic action of killed whole-cell pertussis vaccine prepared from B. pertussis strains 475 and 305. Thymic and splenic lymphocytes of CBA mice 3-7 days following intraperitoneal or intravenous inoculation of pertussis vaccine were shown to inhibit in an antigen-nonspecific manner the plaque-forming cell (PFC) production in the adoptive transfer experiments.

[Mitogenic action of the liquid phase of a microbial suspension of Bordetella pertussis].

The suspension of B. pertussis cells in 0.15 M NaC1 solution, used for the preparation of corpuscular pertussis vaccine contains components loosely bound to microbial cells and producing pronounced mitogenic effect on mouse splenocytes at a concentration of 10 micrograms/ml.

Enhancement of haematopoiesis-directed suppressor activity in mice following Bordetella pertussis vaccine.

A single injection of killed whole-cell pertussis vaccine (strain 5374) into CBA mice (H-2k) was shown to induce a drastic depletion of cells in the thymus accompanied by the enhancement of suppressor T-cell activity. Thymus cells obtained from pertussis vaccine-treated mice were shown to inhibit the endogenous and exogenous colony formation in the spleen by haematopoietic stem cells (CFUs). Treatment of thymus cells with the anti-I-Jk alloantiserum against a specific marker of suppressor T-cells plus complement abolished the inhibitory effect on CFUs proliferation and had a stimulatory action on the capacity of the marrow CFUs to form macroscopic haematopoietic colonies in the spleen as compared to controls.

[Hybridomas synthesizing monoclonal antibodies to Bordetella pertussis toxins].

Hybridomas synthetizing monoclonal antibodies (McAb) to B. pertussis toxin (BPT) and endotoxin, or lipopolysaccharide (LPS), were obtained. The specificity of McAb to BPT was confirmed in the leukocytosis-stimulating factor neutralization test.

[Cloning of the genes of hemolytic factors of Bordetella pertussis in Escherichia coli].

The gene library of B. pertussis strain No. 475 (serovar 1.

[Purification and properties of Bordetella pertussis toxin].

The modified method for the isolation and purification of B. pertussis toxin has been proposed. Chromatography with the use of hydroxylapatite and lentil lectin--Sepharose 4B has permitted the isolation of the preparation purified 600 times.

[Effect of the lymphocytosis-stimulating factor from Bordetella pertussis on murine lymphoid and hemopoietic cells].

Effect of B. pertussis lymphocytosis-promoting factor (LPF) on the lympho-hematopoietic system of mice was studied. The injection of LPF was shown to sharply enhance endogenous colony formation and to induce a severe depletion of thymus cells, reaching its maximum of day 4.

[Isolation of a restriction endonuclease with HindIII-specificity from Bordetella bronchiseptica].

Site-specific restriction endonuclease BbrI has been found in bacteriophage resistant strain B. bronchioseptica 4994. The technique was elaborated for purification of BbrI to the stage free of nuclease and phosphatase contamination.

[Stimulation of the suppressor activity in relation to hematopoietic cells in the mouse thymus as affected by pertussis vaccine].

After a single intraperitoneal injection of formalinized whole-cell pertussis vaccine 5374 into CBA mice a drastic depletion of cells in the thymus accompanied by the enhancement of suppressor T-cells production is observed. T-cells inhibit the endogenous and exogenous colony-formation in the spleen by hematopoietic stem cells (CFUs). Treatment of thymus cells with the anti-I-Jk alloantiserum against a specific marker of suppressor T-cells in the presence of complement removes the inhibitory effect on CFUs proliferation and has a stimulatory action on the capacity of the marrow CFUs to form macroscopic hematopoietic colonies in the spleen compared with control ones.

[Limitation of phage multiplication by microbes of the genus Bordetella].

Possible causes limiting the multiplication of Bordetella phages or inducing their restriction, such as the influence of lysogenic immunity and the restriction-modification (R-M) system or the incompatibility of the receptor apparatus, have been studied. The limitation of the multiplication of phages by some B. bronchiseptica and B.

Changes within the genus Bordetella influenced by phages.

Bordetella phages give rise to changes in the important phenotypic properties of all three Bordetella species. The changes depend on the phage system used. The treatment of Bordetella parapertussis with phages from B.

[Detection of Bordetella bronchiseptica strains containing the main agglutinogens of all species of the genus Bordetella].

The study of 26 B. bronchiseptica strains with typical morphological and biochemical properties resulted in the detection of 8 strains having the main specific agglutinogens of 3 Bordetella species (serovars) in different combinations. The presence of the agglutinogens was confirmed in the agglutination test and the agglutinin adsorption test with the use of monospecific antisera to the main agglutinogens.

[Conversion of Bordetella parapertussis serovar through lysogeny produced by pertussis phages].

Bacteriophages from Bordetella pertussis were titrated on the indicator strain B. parapertussis 17903 by using standard soft agar techniques. Secondary growth, occasionally observed in some phage plaques, was isolated and transferred onto selective media.