A functional mutation associated with piglet diarrhea partially by regulating the transcription of porcine .

The present study aimed to search for functional mutations within the promoter of porcine and to provide causative genetic variants associated with piglet diarrhea. We firstly confirmed that expressed higher in the small intestine than in the spleen, stomach and large intestine of SPF piglets, respectively ( < 0.05).

A 12-bp indel in the 3'UTR of porcine CISH gene associated with Landrace piglet diarrhea score.

Variations in Cytokine inducible SH2-containing protein (CISH) gene influence human susceptibility to common infectious diseases, but little is known about CISH in swine. The objectives of this study were to 1) determine porcine CISH (pCISH) mRNA expression level in different tissues of piglets, 2) predict putative functional genetic variations within pCISH, 3) investigate the association between a identified variation in the 3'UTR and piglets phenotype traits in Min (n = 226) and Landrace (n = 186) population, and explore the function of this variation. Results of quantitative PCR showed pCISH mRNA expressed in all the collected tissues with higher level in lung and ileum than colon (p < 0.

Characterization, mRNA expression profile, subcellular distribution and association analysis with piglet diarrhea of porcine matrix metallopeptidase 7 (pMMP7).

Matrix metalloproteinase 7 (MMP7) is involved in the degradation of extracellular matrix in disease processes and therefore plays an important role in host disease resistance/susceptibility. To better understanding the effects of porcine MMP7 (pMMP7) on piglets diarrhea trait, we characterized pMMP7 gene, identified genetic variations in pMMP7 and explored the relationship between pMMP7 polymorphisms and piglets diarrhea in Min pig and Landrace populations. The complete coding sequence of pMMP7 is 804 bp encoding a protein of 267 amino acids.

Expression pattern and association analysis of porcine matrix metallopeptidase 9 (MMP9) with diarrhea and performance traits in piglets.

Matrix metalloproteinase 9 (MMP9) plays critical roles in multiple biological processes, such as reproduction, cell proliferation and differentiation, and host defenses. The aim of this study was to evaluate whether MMP9 is a candidate gene for resistance to diarrhea in piglets. In this study, quantitative real-time PCR was used to analyze the expression of MMP9 mRNA in different tissues of specific pathogen-free piglets.

Identification of porcine CTLA4 gene polymorphism and their association with piglet diarrhea and performance traits.

The objective of this study was to evaluate the association between the cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) gene and piglet diarrhea. In this study, the mRNA expression of the CTLA4 gene increased significantly in IPEC-J2 cells after Escherichia coli K88 infection. Single nucleotide polymorphisms (SNPs) located in the 5' flanking region (SNPs g.