A new Palaearctic species of the subgenus (Diptera, Tipulidae) from the West Caucasus with a survey of the species group.

The species group in the subgenus Lunatipula is redefined and now consists of five species native to the Caucasus. () is described as new to science, and variations in the male terminalia in two populations are noted. Two subspecies ( and ) are elevated to species rank.

[Molecular, genetic and immunohistochemical investigation of carcinosarcoma of the female reproductive tract].

Tissue precursors and genesis of female reproductive tract carcinoma vis-a-vis its carcinomatous and sarcomatous patterns remain unknown. To determine the clonal origin of 17 female reproductive tract carcinomas, such molecular, genetic and immunohistochemical techniques as PCR-SSCP and/or denaturing gel electrophoresis for K-ras, p53 and PTEN genes; D17S786, CHRNB1, TP53, BAT26 and BAT40 microsatellites and immunostaining for p53 protein were used. Carcinomatous and sarcomatous components were studied separately.

[Dynamic properties of recA protein filaments from E. coli and P. aeruginosa investigated by neutron spin-echo].

Bacterial RecA protein is the key enzyme in the processes of homologous recombination, post-replication repair and induction of SOS-repair functions. While a significant amount of data on the structure of RecA protein and its functional analogs has been obtained, there is little information about the molecular dynamics of this protein. In this work we present the results of neutron spin-echo measurements of the relaxation kinetics of filaments formed by RecA proteins from E.

[Homologous DNA transferase RecA: functional activities and the search for homology by recombining DNA molecules].

Bacterial RecA protein is a prototype of ATP-dependent homologous recombinases found ubiquitously from bacteriophages up to human beings. When RecA filament is forming on single-stranded DNA in the presence of ATP, it initiates the strand exchange reaction with homologous double-stranded DNA. Among three phases of the reaction (the search for homology, the three-stranded structure annealing in conjunction with the switch of pairing, and the strand displacement) the first one is the most enigmatic and least studied.

[Identification of Rad51 protein from Chlamydomonas reinhardtii: recombinational characteristics].

The unicellular green microalga Chlamydomonas reinhardtii is a perspective model object for basic and applied research. However, its homologous recombination (HR) system which lies in the basis of double-strand DNA break repair have still not been studied. Last years the program of C.

[Damage to the transforming growth factor TGF-beta type II receptor gene and microsatellite instability in carcinoma cells of the gastrointestinal tract].

In order to compare the frequency of damage to the transforming growth factor TGF-beta receptor type II gene (RII gene) and microsatellite instability (MIN) in oncogenesis of sporadic and hereditary cancer of gastrointestinal tract (GIT), 4 groups of carcinomas were analyzed. They included sporadic gastric (GC), family gastric (FGC), sporadic colorectal (CC) and hereditary nonpolyposis colorectal (HNPCC) carcinomas having appropriate clinical and pathological characteristics. Each group consisted of two types of carcinomas, one of them showing MIN.

[Diagnostic values of polymerase chain reaction test in females with genital tuberculosis].

The previously offered procedure for detecting. Mycobacterium tuberculosis, which is based on the polymerase chain reaction (PCR), has been tested for diagnosing genital tuberculosis in females. PCR was used to examine endometrial curettage specimens in 44 patients with different nosological entities, which showed a high sensitivity (80%) and a high specificity in the diagnosis of genital tuberculosis.

[Recombination properties of the modified Pseudomonas aeruginosa RecA protein].

Gene recA from Pseudomonas aeruginosa was cloned into pUC19 vector under lacZ promoter. The expressed protein appeared to be modified, the aminoterminal part of deduced amino acid sequence of the RecAPa protein was found elongated by a polypeptide of 10 amino acids. The modified protein named RecA*Pa completely replaces RecAEc from E.

[Two types of DNA-pairing enzymatic activities detected in rat spermatocyte nuclei: partial purification and characteristics].

Two types of different activity that can promote homologous pairing and strand transfer between suitable DNA substrates have been partially purified from nuclear extracts of rat testes and characterized. Two activities are separated by chromatography on a ssDNA-cellulose column. One activity promotes strand transfer between single-strand circular phage and duplex fragment DNA (346 or 943 bp).

[The recombination mechanism for precise excision of the IS50 mobile element in Escherichia coli K12 cells].

Precise excision of composite transposons of Tn10 type, with long inverted repeats, proceeds according to the slippage mechanism (Brunier et al. parallel Cell. 1988.