Development of miniature base editors using engineered IscB nickase.

As a miniature RNA-guided endonuclease, IscB is presumed to be the ancestor of Cas9 and to share similar functions. IscB is less than half the size of Cas9 and thus more suitable for in vivo delivery. However, the poor editing efficiency of IscB in eukaryotic cells limits its in vivo applications.

A phage cocktail combined with the enteric probiotic ameliorated mouse colitis caused by .

serovar Typhimurium () is one of the most important foodborne pathogens that causes colitis in humans. In this study, we compared the effects of a therapeutic treatment using a phage cocktail (Pc) in combination or not with () in an -induced colitis murine model. An oral administration of 4 × 10 CFU per mouse of resulted in intestinal barrier disruption and severe inflammatory symptoms.

Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype .

() is an important nosocomial and community acquired opportunistic pathogen which causes various infections. The emergence of multi-drug resistant (MDR) and carbapenem-resistant hypervirulent (CR-hvKP) has brought more severe challenge to the treatment of infection. In this study, a novel bacteriophage that specifically infects was isolated and named as vB_KpnM_P-KP2 (abbreviated as P-KP2).

Bacteriophage Protects Against Infection in a Murine Mastitis Model.

Bovine mastitis, an inflammatory disease that occurs frequently in early lactation or the dry period, is primarily caused by bacterial infections. There is growing evidence that () is becoming an important cause of bovine mastitis. The treatment of bovine mastitis is primarily based on antibiotics, which not only leads to a large economic burden but also the development of antibiotic resistance.

Therapeutic Efficacy of Phage P SAE-01E2 against Abortion Caused by Salmonella enterica Serovar Abortusequi in Mice.

subsp. serovar Abortusequi is a frequently reported pathogen causing abortion in mares. In this study, the preventive and therapeutic effects of phage P SAE-01E2 against Abortusequi in a mouse model of abortion were investigated.

The Yersinia Phage X1 Administered Orally Efficiently Protects a Murine Chronic Enteritis Model Against Infection.

is generally considered an important food-borne pathogen worldwide, especially in the European Union. A lytic Yersinia phage X1 (Viruses; dsDNA viruses, no RNA stage; Caudovirales; and Myoviridae) was isolated. Phage X1 showed a broad host range and could effectively lyse 27/51 strains covering various serotypes that cause yersiniosis in humans and animals (such as serotype O3 and serotype O8).