Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens.

The objective of this study was to investigate the effect of sorghum with or without protease and NSP-ase on performance, jejunal histomorphology, caecal short-chain fatty acids (SCFA), and gene expression of barrier function in the broiler chickens. A total of 240 males New Lohmann strain MB 202 broiler chicks (1-day-old) were randomly placed into 12 treatments with 4 replications, 5 birds per replicate for 35 d. The diets were a factorial combination of 3 levels of sorghum (0, 10, and 20%), 2 concentrations of protease (with and without protease), and 2 concentrations of NSP-ase (with and without NSP-ase).