Analysis of chromatin accessibility in decidualizing human endometrial stromal cells.

Spontaneous decidualization of the endometrium in response to progesterone signaling is confined to menstruating species, including humans and other higher primates. During this process, endometrial stromal cells (EnSCs) differentiate into specialized decidual cells that control embryo implantation. We subjected undifferentiated and decidualizing human EnSCs to an assay for transposase accessible chromatin with sequencing (ATAC-seq) to map the underlying chromatin changes.

Survival of Escherichia coli after isoelectric solubilization and precipitation of fish protein.

Protein recovery for fish processing by-products utilizes extreme pH shifts for isoelectric solubilization and precipitation. The purpose of this study was to determine if Escherichia coli would survive exposure to the extreme pH shifts during the protein recovery process. Fresh rainbow trout were beheaded, gutted, and minced and then inoculated with approximately 10(9) CFU of E.

Survival of Listeria innocua after isoelectric solubilization and precipitation of fish protein.

Protein wasted by the disposal of fish processing by-products may be recovered using isoelectric solubilization and precipitation. Extreme pH shifts are used to solubilize the protein and then it is recovered by precipitation and centrifugation. Microbial survival after this process is unknown; therefore, the purpose was to see if Listeria innocua would survive extreme pH shifts during the protein recovery process.

Temporary atrial pacing after cardiac surgery.

Atrial epicardial wires are used after cardiac surgery for diagnostic and therapeutic purposes. Postoperative dysrhythmias may be diagnosed by using an atrial wire electrogram, and treatment of these dysrhythmias includes atrial pacing. In this article atrial pacing during the post-cardiac surgery period is discussed in relation to the management of supraventricular dysrhythmias, bradydysrhythmias, ventricular dysrhythmias, and low cardiac output.

Cardiac care. Signal-averaged electrocardiograms.

The signal-averaged electrocardiogram (ECG) is a simple, noninvasive test used to predict which patients may be prone to sudden cardiac death because of ventricular tachycardia. Signal averaging allows detection of low-amplitude waveforms in the ECG that are normally masked by noise. The averaging process is done by a computerized ECG machine.