Preparation of disulfiram-Cu-polylactide nanofibrous membranes via electrostatic spinning and evaluation of their in vitro anticancer effects.

The antitumor effects of disulfiram (DSF) -a conventional medication used to treat alcohol dependence-have been documented in numerous studies. However, because of its low water solubility and Cu-dependent anticancer effects, the application of DSF in cancer therapy has been limited. Nanofibrous membranes produced via electrospinning have large specific surface areas.

Atomic force microscopy wide-field scanning imaging using homography matrix optimization.

During the offline combination of multiple atomic force microscopy (AFM) images, changes in probe displacement can be affected by dynamic noise, leading to dislocation and tearing in the stitching. To overcome this, we designed a homography matrix optimization method to describe the relative positional relationship between images by constructing the original image matrix and applying singular value decomposition to denoise the homography matrix. Additionally, we implemented a scale-invariant feature transform (SIFT) to extract feature points.

Mechanism of action and side effects of colchicine based on biomechanical properties of cells.

Many diseases are related to changes in the biomechanical properties of cells; their study can provide a theoretical basis for drug screening and can explain the internal working of living cells. In this study, the biomechanical properties of nephrocytes (VERO cells), hepatocytes (HL-7702 cells), and hepatoma cells (SMCC-7721 cells) in culture were detected by atomic force microscopy (AFM) to analyse the side effects of colchicine at different concentrations (0.1 μg/mL (A) and 0.

Electrical conductivity measurement ofDNA molecules by conductive atomic force microscopy.

Conductive atomic force microscopy (C-AFM) is a powerful tool used in the microelectronics analysis by applying a certain bias voltage between the conducting probe and the sample and obtaining the electrical information of sample. In this work, the surface morphological information and current images of the lambda DNA (DNA) molecules with different distributions were obtained by C-AFM. The 1 and 10 nglDNA solutions were dripped onto mica sheets for making randomly distributed DNA and DNA network samples, and another 1 nglDNA sample was placed in a DC electric field with a voltage of 2 V before being dried for stretching the DNA sample.

Analysis of Colchicine-Induced Effects on Hepatoma and Hepatocyte Cells by Atomic Force Microscopy.

Biomechanical properties of cells are altered by many diseases. Cancer cell metastasis is related to the properties such as the cell stiffness that influences cell proliferation, differentiation and migration. In this paper, we used an atomic force microscope to analyze the colchicine-induced effects on the mechanical properties of hepatocyte (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture at the nanoscale.

Axisymmetric Contact Problem for a Flattened Cell: Contributions of Substrate Effect and Cell Thickness to the Determination of Viscoelastic Properties by Using AFM Indentation.

Nanoindentation technology has proven to be an effective method to investigate the viscoelastic properties of biological cells. The experimental data obtained by nanoindentation are frequently interpreted by Hertz contact model. However, in order to validate Hertz contact model, some studies assume that cells have infinite thickness which does not necessarily represent the real situation.

Biomechanical measurement and analysis of colchicine-induced effects on cells by nanoindentation using an atomic force microscope.

Colchicine is a drug commonly used for the treatment of gout, however, patients may sometimes encounter side-effects induced by taking colchicine, such as nausea, vomiting, diarrhea and kidney failure. In this regard, it is imperative to investigate the mechanism effects of colchicine on biological cells. In this paper, we present a method for the detection of mechanical properties of nephrocytes (VERO cells), hepatocytes (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture by atomic force microscope (AFM) to analyze the 0.