Glass nano/micron pipette-based ion current rectification sensing technology for single cell/ analysis.

Glass nano/micron pipettes, owing to their easy preparation, unique confined space at the tip, and modifiable inner surface of the tip, can capture the ion current signal caused by a single entity, making them widely used in the construction of highly sensitive and highly selective electrochemical sensors for single entity analysis. Compared with other solid-state nanopores, their conical nano-tip causes less damage to cells when inserted into them, thereby becoming a powerful tool for the analysis of important substances in cells. However, glass nanopipettes have some shortcomings, such as poor mechanical properties, difficulty in precise preparation (aperture less than 50 nm), and easy blockage during complex real sample detection, limiting their practicability.

Enhanced Electrochemical Performance and Cycling Stability of the (NH)[VVO(OH)]·11HO Cathode in Aqueous Zinc Ion Batteries.

Although vanadium-based compounds possess several advantageous characteristics, such as multivalency, open structure, and high theoretical specific capacity, which render them highly promising candidates for cathode materials in aqueous zinc ion batteries (AZIBs), their large-scale application still necessitates addressing the challenges posed by slow kinetics resulting from low conductivity and capacity degradation caused by material dissolution. Therefore, we have successfully synthesized high-purity mixed multivalent (NH)[VVO(OH)]·11HO (NVO) crystalline materials via a liquid-phase precipitation modulation method and employed it as an innovative AZIB cathode material for the first time. It exhibits a remarkable reversible specific capacity of 240 and 102.

Antibacterial Activity and Action Mechanism of Bacteriocin Paracin wx7 as a Selective Biopreservative against Vancomycin-Resistant in Fresh-Cut Lettuce.

Fresh-cut vegetables are widely consumed, but there is no food preservative available to selectively inhibit vancomycin-resistant , which is a serious health menace in fresh-cut vegetables. To develop a promising food biopreservative, a bacteriocin, paracin wx7, was synthesized, showing selective inhibition against with MIC values of 4-8 μM. It showed instant bactericidal mode within 1 h at high concentrations with concomitant cell lysis against vancomycin-resistant .

Controlling soft rot of green pepper by bacteriocin paracin wx3 and its effect on storage quality of green pepper.

A bacteriocin paracin wx3 was investigated as a candidate of natural preservative to control green pepper soft rot. Firstly, paracin wx3 was heterologously expressed in Pichia pastoris X33 with an improved yield of 0.537 g/L.

FeP-FeO nanospheres for electrocatalytic N reduction to NH under ambient conditions.

The electrocatalytic nitrogen reduction reaction (eNRR) under ambient conditions is deemed a promising alternative for NH synthesis. In this paper, an FeP-FeO nanocomposite electrocatalyst was prepared by phosphating annealing using FeO as a precursor, and the resulting FeP-FeO exhibited excellent N-to-NH-producing activity over a wide potential window. The highest faradaic efficiency of FeP-FeO is 11.

MOF-Derived Bimetallic Selenide CoNiSe Nanododecahedrons Encapsulated in Porous Carbon Matrix as Advanced Anodes for Lithium-Ion Batteries.

Transition metal selenides have received considerable attention as promising candidates for lithium-ion battery (LIB) anode materials due to their high theoretical capacity and safety characteristics. However, their commercial viability is hampered by insufficient conductivity and volumetric fluctuations during cycling. To address these issues, we have utilized bimetallic metal-organic frameworks to fabricate CoNiSe/C nanodecahedral composites with a high specific surface area, abundant pore structures, and a surface-coated layer of the carbon-based matrix.

Deformable Catalytic Material Derived from Mechanical Flexibility for Hydrogen Evolution Reaction.

Deformable catalytic material with excellent flexible structure is a new type of catalyst that has been applied in various chemical reactions, especially electrocatalytic hydrogen evolution reaction (HER). In recent years, deformable catalysts for HER have made great progress and would become a research hotspot. The catalytic activities of deformable catalysts could be adjustable by the strain engineering and surface reconfiguration.

Methanol-water mixture evaporation-induced self-assembly of ZIF-8 particles.

We report a strategy for the self-assembly of zeolitic imidazolate framework-8 (ZIF-8) particles induced by the evaporation of a methanol-water mixture. This strategy effectively suppresses the coffee ring effect, facilitates the rapid assembly of ZIF-8 particles, and improves the orientation and optical properties of self-assembled superstructures.

Soft rot of postharvest pepper: bacterial pathogen, pathogenicity and its biological control using Lactobacillus farciminis LJLAB1.

Background: Soft rot is the most important bacterial disease of postharvest pepper during storage and transportation. The main objectives of this study were to investigate the bacterial pathogen species causing pepper soft rot and seek for an antagonistic bacterium to control this disease.

Results: Pathogens Pectobacterium carotovorum, Enterobacter sp.

Hydrogen-bonded organic framework-stabilized charge transfer cocrystals for NIR-II photothermal cancer therapy.

Charge-transfer (CT) cocrystals consisting of an electron donor and acceptor have gained attention for designing photothermal (PT) conversion materials with potential for biomedical and therapeutic use. However, the applicability of CT cocrystals is limited by their low stability and aqueous dispersity in biological settings. In this study, we present the self-assembly of CT cocrystals within hydrogen-bonded organic frameworks (HOFs), which not only allows for the dispersion and stabilization of cocrystals in aqueous solution but also promotes the CT interaction within the confined space of HOFs for photothermal conversion.

Colorimetric Aerogel Gas Sensor with High Sensitivity and Stability.

The detection of formic acid vapor in the usage environment is extremely important for human health and safety. The utilization of metal-organic frameworks (MOFs) for the detection of gaseous molecules is an attractive strategy. However, the rational design and construction of MOF-based gas sensors with high sensitivity and mechanical stability remain a significant challenge.

Solid-State Nanopore/Nanochannel Sensing of Single Entities.

Solid-state nanopores/nanochannels, with their high stability, tunable geometry, and controllable surface chemistry, have recently become an important tool for constructing biosensors. Compared with traditional biosensors, biosensors constructed with solid-state nanopores/nanochannels exhibit significant advantages of high sensitivity, high specificity, and high spatiotemporal resolution in the detection single entities (such as single molecules, single particles, and single cells) due to their unique nanoconfined space-induced target enrichment effect. Generally, the solid-state nanopore/nanochannel modification method is the inner wall modification, and the detection principles are the resistive pulse method and the steady-state ion current method.

A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork.

The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all l-arginine residues of a reported peptide (IIRR)-NH (zp80) were substituted with the corresponding D enantiomers.

Phenylalanine Promotes Biofilm Formation of to Improve Biocontrol Efficacy against Jujube Black Spot Rot.

During storage and transportation after harvest, the jujube fruit is susceptible to black spot rot, which is caused by . The present study aimed to evaluate the effectiveness of the yeast in controlling in postharvest jujube fruits, and to explore the biofilm formation mechanism. The results showed that treatment significantly reduced the decay in jujube fruits.

A core epiphytic bacterial consortia synergistically protect citrus from postharvest disease.

Biological antagonists are a series of microbes that can control pathogens to reduce the incidence of disease or reduce symptoms. Herein, four varieties of citrus fruit were selected to perform an amplicon sequencing on their epiphytic microbiota to get a systematic understanding of them. Co-occurrence network, Venn, and LefSe analysis were performed to filter to 24 genera which represent the universality, specificity, and correlation among samples.

Tryptophan enhances biocontrol efficacy of Metschnikowia citriensis FL01 against postharvest fungal diseases of citrus fruit by increasing pulcherriminic acid production.

The aim of this work was to study the capability and mechanism of enhancing the yield of pulcherriminic acid (PA) produced by Metschnikowia citriensis FL01 with the help of tryptophan for the control of postharvest diseases on citrus caused by Penicillium italicum, Geotrichum citri-aurantii and Penicillium digitatum. The adding of 10 mmol/L tryptophan to the growth medium resulted in the widest pulcherrimin pigment zone produced by M. citriensis FL01.

C-terminal modification of a de novo designed antimicrobial peptide via capping of macrolactam rings.

In this work, by capping a macrolactam ring at the C-terminus of a de novo-designed peptide, namely zp80, we have constructed a small peptide library via the solid phase peptide synthesis for screening. Eight peptides bearing different aspartic acid-rich macrolactam rings but the same linear (IIRR) unit exhibited improved antibacterial activities, hemolytic activity, and selectivity index. Mechanistic studies revealed that they could destroy the integrity of bacterial envelope, leading to cytoplasm leakage and rapid dissipation of membrane potential.

Debaryomyces nepalensis reduces fungal decay by affecting the postharvest microbiome during jujube storage.

Microbial antagonists are effective and environmentally friendly in controlling postharvest diseases of fruit. The present study investigated the influence of D. nepalensis on epiphytic microbiome and postharvest decay of jujube.

Low and high storage temperature inhibited the coloration of mandarin fruit (Citrus unshiu Marc.) with different mechanism.

Background: Peel color regulated by pigment metabolism is one of the most crucial indicators affecting the commodity values of citrus fruit. Storage temperature is a vital environmental factor that regulates the fruit pigmentation.

Results: Results showed that the peel coloring process was significantly inhibited when mandarin fruit were stored at 5 and 32 °C with normal coloring at 25 °C as the control.

Influence of arginine on the biocontrol efficiency of Metschnikowia citriensis against Geotrichum citri-aurantii causing sour rot of postharvest citrus fruit.

This study investigated the effect of arginine (Arg) on the antagonistic activity of Metschnikowia citriensis against sour rot caused by Geotrichum citri-aurantii in postharvest citrus, and evaluated the possible mechanism therein. Arg treatment up-regulated the PUL genes expression, and significantly induced the pulcherriminic acid (PA) production of M. citriensis, which related to the capability of iron depletion of M.

Metschnikowia citriensis FL01 antagonize Geotrichum citri-aurantii in citrus fruit through key action of iron depletion.

Metschnikowia citriensis FL01 has great potential for biocontrol applications for its excellent biocontrol efficacy on postharvest diseases of citrus fruit, and the iron depletion by pulcherriminic acid (PA) and then formation of insoluble pigment pulcherrimin had been speculated as an important action mechanism. To identify the genes involved in pulcherrimin synthesis and reutilization in M. citriensis FL01, we de novo assembled the genome of M.

Transformation of Inferior Tomato into Preservative: Fermentation by Multi-Bacteriocin Producing WX322.

Loss and waste of postharvest vegetables are the main challenges facing the world's vegetable supply. In this study, an innovative method of value-added transformation was provided: production of bacteriocin from vegetable waste, and then its application to preservation of vegetables. Antibacterial activity to soft rot pathogen ( BZA12) indicated that tomato performed best in the nutrition supply for bacteriocin production among 12 tested vegetables.

An ornithine-rich dodecapeptide with improved proteolytic stability selectively kills gram-negative food-borne pathogens and its action mode on Escherichia coli O157:H7.

Food-borne pathogenic bacteria are dispersed throughout the entire chain of the food industry. However, many food preservatives are limited by poor biocompatibility such as cumulative poisoning. The antimicrobial peptide is increasingly regarded as a promising preservative in food research due to its high bioactivity and low cytotoxicity.

Investigation of antibiofilm activity, antibacterial activity, and mechanistic studies of an amphiphilic peptide against Acinetobacter baumannii.

Biofilm-producing pathogens, such as Acinetobacter baumannii, have aroused escalating attention. Because these bacteria could secrete mixture with close-knit architecture and complicated components to resist traditional antibiotics. Here, we reported an amphiphilic peptide denoted as zp3 (GIIAGIIIKIKK-NH), which showed favorable bioactivity against Acinetobacter baumannii ATCC 19606 (minimal inhibitory concentration, MIC = 4 μM) and low cytotoxicity to mammalian cells Vero (half maximal inhibitory concentration, IC > 100 μM).