J Integr Plant Biol
January 2009
The endopeptidases (EPs) in wheat endosperm during seed germination and subsequent seedling growth were characterized by gradient-polyacrylamide gel electrophoresis with gelatin copolymerized into the gel. Four cysteine EPs (EP1, EP2, EP3 and EP4) were detected in wheat endosperm during the 7 d growth after seed imbibition. The results also showed that the activities of all of these EPs increased continuously, and EP2 first appeared and had the highest proteolytic activity among the four EPs in this experimental process.
View Article and Find Full Text PDFThe degradation of large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in wheat (Triticum aestivum L. cv. Yangmai 158) leaves was studied.
View Article and Find Full Text PDFZhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
October 2006
The role of serine endopeptidase in cucumber leaf senescence was studied by using the inhibitor of serine endopeptidase and plant growth regulators (6-BA and ABA) on darkness-induced cucumber leaves. The results showed that the senescence of cucumber leaves were delayed by AEBSF [4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride], an inhibitor of serine-type endopeptidase, or 6-BA treatment. The chlorophyll contents increased by AEBSF (Fig.
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
March 2006
Human brain natriuretic peptide (hBNP) was used clinically for the treatment of acute decompensated congestive heart failure. In this paper, hBNP was expressed as a fusion protein with a histidine tag and Ssp dnaB mini-intein which was capable of self-cleavage. After affinity chromatography with Ni-Sepharose and renaturation, the fusion protein was enriched with CM-cellulose.
View Article and Find Full Text PDFZhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
June 2005
Results in this paper showed that the effect of nitric oxide (NO) donor, sodium nitroprusside (SNP), which could promote the germination of wheat seeds under osmotic stress, was through enhancing imbibition using water content determination and imbibition analysis of wheat seed. SNP could also enhance amylase isozyme I activities, accelerate the liquefaction of endosperms, while have no effect on esterase. Moreover, SNP could strongly increase the sugars, such as the fructose, glucose and sucrose contents in wheat seeds during early germination (12 h) under normal conditions.
View Article and Find Full Text PDFZhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
October 2004
Effects of nitric oxide (NO), a substance newly found to have protective functions in plants, on root growth of wheat (Triticum aestivum L. Yangmai 158) seedlings under salt stress were studied. Sodium nitroprusside (SNP), an NO donor, markedly alleviated the inhibitory effect of salt on root elongation at salt concentrations around 150 mmol/L, but was ineffective when NaCl concentration was at 300 mmol/L or higher.
View Article and Find Full Text PDFZhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
April 2004
When wheat (Triticum aestivum L. Yangmai 158) seedling (with three fully expanded leaves) roots were treated with 15% PEG-6000 in combination with different concentrations (0.1 and 0.
View Article and Find Full Text PDFThe effects of nitric oxide (NO) and gibberellic acid (GA(3)) on the responses of amylases in wheat (Triticum aestivum L.) seeds (caryopses) were investigated during the first 12 h of germination. GA(3) had no effects on the activities of alpha-amylase (EC 3.
View Article and Find Full Text PDFThe contribution of rice as a protein source is important. Rice seed protein can be divided into four forms, glutelin (57 kDa, 37-39 kDa, 22-23 kDa), prolamine (13 kDa), albumin (16 kDa) and globulin (10 kDa, 26 kDa) on its solubility. Glutelin is the major storage protein of rice and accounted for 80% of total protein found in the rice grain, the mature glutelin comprises an acidic (37-39 kDa) and an basic subunit (22-23 kDa) coming from a common precursor (57 kDa) by post-transcriptional hydrolytic cleavage.
View Article and Find Full Text PDFAluminum-induced exudation of organic acids from roots has been proposed as a mechanism for Al tolerance in plants. To better understand the regulatory process leading to efflux of organic acids, the possible involvement of salicylic acid (SA) in regulating Al-induced citrate release in Cassia tora L. was identified.
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