Ligation of the intersphincteric fistula tract (LIFT) for high transsphincteric fistulas: a double-center retrospective study with long-term follow-up.

Purpose: This study aimed to evaluate the long-term efficacy of the ligation of the intersphincteric fistula tract (LIFT) procedure in treating high transsphincteric fistulas.

Methods: We conducted a retrospective study to evaluate the success rate of LIFT treatment in 82 patients with high transsphincteric fistulas involving at least 1/3 of the external sphincter. This study was carried out across 2 centers from November 2009 to February 2023.

Kuntai Capsule Improves Ovarian Function in Rats with Premature Ovarian Failure After Transplantation of Cryopreserved Ovarian Tissue by Regulating Sex Hormones and Apoptosis.

Objective: Our study aimed to investigate the therapeutic effects of the Kuntai capsule in improving ovarian function in rats with transplantation of cryopreserved ovary.

Methods: Two mice ovary cell lines were cultured with Kuntai capsule decoction, and cell apoptosis was detected by MTT assay. A total of 90 SPF Sprague Dawley rats were included in this study.

N influences on CH accumulation and displacement in shale by molecular dynamics.

N is generally employed as a displacement agent to enhance gas recovery in shale gas-bearing reservoirs. However, the primary displacement mechanism in the subsurface still needs to be clarified due to the characteristics of shale reservoirs with low porosity and abundant nanopores. This study employs the Molecular Dynamics (MD) simulation method to investigate the effects of N on the CH accumulation and displacement processes by adopting practical conditions in the subsurface environment.

Simultaneous Anti-Tuberculosis and Anti-Tumor Treatment with Immune Checkpoint Inhibitors for Co-Existent Pulmonary Tuberculosis and Advanced Lung Cancer.

Background: Immune checkpoint inhibitors (ICIs) have emerged as the first-line treatment for driver-negative advanced non-small cell lung cancer (NSCLC). However, there is uncertainty regarding the availability and timing of ICI initiation in patients with NSCLC combined with pulmonary tuberculosis (TB). Additionally, the implementation of dual therapy for anti-TB and anti-tumor treatment poses significant challenges in terms of avoiding drug-drug interactions and reducing adverse reactions during clinical diagnosis and treatment.

Aurora kinase B inhibitor AZD1152: repurposing for treatment of lupus nephritis driven by the results of clinical trials.

Background: Lupus nephritis (LN) is one of the most common and severe complications of systemic lupus erythematosus (SLE). Multitarget therapy (MT) achieves a 20% higher complete remission (CR) rate compared to conventional therapy in LN management. Intrigued by its excellent clinical efficacy, we aimed to develop a single-agent therapy with comparable efficacy to MT, offering a simplified treatment regimen.

Transparent and Antifouling Lubrication Coating for Medical Applications Formed by a Self-Assembled Method.

Lubrication surfaces reduce the risk of cross-contamination and enhance the long-term stability of medical devices, which holds significance in the realm of antifouling medical materials. However, the complexity of constructing micronano structures to immobilize lubricating fluids and the fluorine content typically found in silane coupling agents restrict their widespread adoption. In this study, we prepared a biomimetic lubricating coating (BLC) through the one-step self-assembly of octadecyltrichlorosilane and oil infusion.

Clinical characteristics and repositioning efficacy analysis of posterior canal-benign paroxysmal positional vertigo-canalolithiasis and vertigo-cupulolithiasis patients.

Background: Currently, there is a ‌paucity of research‌ comparing the clinical characteristics and repositioning efficacy between ‌posterior canal-benign paroxysmal positional vertigo-canalolithiasis (PC-BPPV-ca)‌ and ‌posterior canal-benign paroxysmal positional vertigo-cupulolithiasis (PC-BPPV-cu)‌.

Aims/objectives: To observe the clinical characteristics and compare the efficacy of repositioning maneuvers between PC-BPPV-ca and PC-BPPV-cu patients.

Material And Methods: Clinical information of patients was collected.

Construction of a Macrophage-Tropic Subtype C HIV-1-mGreenLantern Reporter Virus for Studies on HIV-1 Replication and the Impact of Methamphetamine.

HIV-1 subtype C viruses are responsible for 50% of global HIV burden. However, nearly all currently available reporter viruses widely used in HIV research are based on subtype B. We constructed and characterized a replication-competent HIV-1 subtype C reporter virus expressing mGreenLantern.

Phycocyanin-based multifunctional microspheres for treatment of infected radiation-induced skin injury.

Radiation therapy is a primary modality for cancer treatment; however, it often leads to various degrees of skin injuries, ranging from mild rashes to severe ulcerations, for which no effective treatments are currently available. In this study, a multifunctional microsphere (PC@CuS-ALG) was synthesized by encapsulating phycocyanin-templated copper sulfide nanoparticles (PC@CuS) within alginate (ALG) using microfluidic technology. Phycocyanin, a natural protein derived from microalgae, shows abilities to scavenge reactive oxygen species, repair radiation-induced damage to skin cells, and ameliorate macrophage-related inflammatory responses.

Short-term efficacy of right-to-left shunt closure in patients with vestibular migraine.

Objective: This study aims to evaluate the short-term efficacy of right-to-left shunt closure in vestibular migraine patients, and compare the efficacy between patent foramen ovale (PFO) closure and pulmonary arteriovenous malformation (PAVM) embolization. Additionally, the study identifies factors related to surgical outcomes.

Methods: Forty-one patients with vestibular migraine and medium to large right-to-left shunts underwent surgery: PFO closure, PAVM embolization, or both.

An analysis of meteorological and environmental factors linked to Thunderstorm Asthma in China.

Background: Thunderstorm Asthma (TA) events are observed worldwide, but the precise triggering mechanisms remain elusive.

Objective: This study aims to outline the environmental patterns associated with TA events in China.

Methods: Environmental data was collected from Chinese cities that have experienced TA events, focusing on meteorological conditions in the seven days preceding the thunderstorms.

Predictive value of geriatric nutritional risk indexes for hospital readmission and mortality in older patients.

The Geriatric Nutritional Risk Index (GNRI) plays a crucial role in assessing nutritional status and predicting clinical outcomes in older patients. This study explores the GNRI as a prognostic tool for clinical outcomes in older inpatients. Spanning from August 2013 to December 2020, the research involved 573 older patients at Tianjin Third Central Hospital, China.

HSP90 stabilizes visual cycle retinol dehydrogenase 5 in the endoplasmic reticulum by inhibiting its degradation during autophagy.

Genetic mutations in retinol dehydrogenase 5 (RDH5), a rate-limiting enzyme of the visual cycle, is associated with nyctalopia, age-related macular disease, and stationary congenital fundus albipunctatus (FA). A majority of these mutations impair RDH5 protein expression and intracellular localization. However, the regulatory mechanisms underlying RDH5 metabolism remain unclear.

Prognostic Value of N1/N2 Neutrophils Heterogeneity and Tertiary Lymphoid Structure in Hepatocellular Carcinoma Patients.

Background: The tumor immune microenvironment, including neutrophils and tertiary lymphoid structures (TLSs), is pivotal for HCC prognosis assessment. Tumor-associated neutrophils exhibit plasticity, adopting either an antitumorigenic N1 (MPO+ CD206-) or a pro-tumorigenic N2 (MPO+ CD206+) phenotype. We explored the prognostic value of neutrophil plasticity and TLS maturity in HCC in both tumor and peritumoral tissues and addressed their interaction.

Prognostic significance of NUAK1 and its association with immune infiltration in stomach adenocarcinoma.

Background: Stomach adenocarcinoma (STAD) represents a significant global health burden, accounting for a considerable proportion of cancer-related mortalities, and NUAK1, a protein kinase, plays a crucial role in cellular metabolism, cell cycle regulation, migration, and tumor progression. However, its relationship with prognosis and immune infiltration in STAD has not been thoroughly investigated.

Methods: RNA sequencing data from the Cancer Genome Atlas (TCGA) and Genotypic Tissue Expression Project (GTEx) databases were employed to assess disparities in NUAK1 expression between STAD tumour and normal tissues.

Immature PIT1-lineage Pituitary Neuroendocrine Tumors/Adenomas, a Morphologically Unique Pituitary Neuroendocrine Tumors/Adenomas Commonly With Cytologic Atypia Features and a Predilection for Aggressive Clinical Potential.

Immature PIT1-lineage pituitary neuroendocrine tumors (PitNETs)/adenomas (Immature PIT1-lineage tumors) are a rare and underrecognized subtype of PitNETs that exhibits distinct cytologic atypia features and aggressive clinical potential. This study characterizes the clinical, radiological, histologic, and immunohistochemical features of 15 immature PIT1-lineage tumors identified from 1084 PitNETs patients over 5 years. Our cohort of 6 males and 9 females had a median age of 37.

Role of mitophagy in spinal cord ischemia-reperfusion injury.

Spinal cord ischemia-reperfusion injury, a severe form of spinal cord damage, can lead to sensory and motor dysfunction. This injury often occurs after traumatic events, spinal cord surgeries, or thoracoabdominal aortic surgeries. The unpredictable nature of this condition, combined with limited treatment options, poses a significant burden on patients, their families, and society.

Long-term aerobic exercise improves learning memory capacity and effects on oxidative stress levels and Keap1/Nrf2/GPX4 pathway in the hippocampus of APP/PS1 mice.

Objective: To examine the effects of long-term aerobic exercise on oxidative stress and learning memory ability of APP/PS1 mice, focusing on the hippocampal Keap1, Nrf2, HO-1, and GPX4 proteins.

Methods: Thirty APP/PS1 double transgenic AD mice were randomly divided into three groups: model group, short-term exercise group, and long-term exercise group, with 10 mice in each group. Male non-transgenic mice of the same age served as the control group.

The interaction of BDNF with estrogen in the development of hypertension and obesity, particularly during menopause.

The expression of BDNF in both neuronal and non-neuronal cells is influenced by various stimuli, including prenatal developmental factors and postnatal conditions such as estrogens, dietary habits, and lifestyle factors like obesity, blood pressure, and aging. Central BDNF plays a crucial role in modulating how target tissues respond to these stimuli, influencing the pathogenesis of hypertension, mitigating obesity, and protecting neurons from aging. Thus, BDNF serves as a dynamic mediator of environmental influences, reflecting an individual's unique history of exposure.

A novel linear B cell epitope of the porcine circovirus type 3 capsid protein identified by phage display technology.

Porcine circovirus type 3 (PCV3) is endemic in swine worldwide and causes reproductive disorders, dermatitis and nephrotic syndrome, and multi-organ inflammation. PCV3 capsid protein (Cap) can self-assemble into virus-like particles (VLPs), and is an ideal candidate for vaccines and diagnostic reagents.In this study, the recombinant PCV3 Cap protein was successfully expressed in E.