A R2R3-MYB transcription factor, FeR2R3-MYB, positively regulates anthocyanin biosynthesis and drought tolerance in common buckwheat (Fagopyrum esculentum).

The R2R3-MYB transcription factors (TFs) play a crucial role in regulating plant secondary metabolism and abiotic stress responses, yet they are still poorly understood in common buckwheat (Fagopyrum esculentum), a valuable minor grain crop resource. In this study, a candidate gene, FeR2R3-MYB, was cloned from the anthocyanin-rich common buckwheat variety 'QZZTQ'. FeR2R3-MYB was found to contain two MYB DNA-binding domains and be located at the nucleus with transcriptional activation activity.

TMT-based comparative proteomics reveals the role of acyl-CoA oxidase 4 in enhancing the drought stress tolerance in common buckwheat (Fagopyrum esculentum).

Drought stress has been the main abiotic factor affecting the growth, development and production of common buckwheat (Fagopyrum esculentum). To explore the response mechanisms of regulating buckwheat drought stress on the post-transcriptional and translational levels, a comparative proteomic analysis was applied to monitor the short-term proteomic variations under the drought stress in the seedling stage. From which 593 differentially abundant proteins (DAPs) were identified using the TMT-based proteomics analysis.