Kynurenic Acid Accelerates Healing of Corneal Epithelium In Vitro and In Vivo.

Kynurenic acid (KYNA) is an endogenous compound with a multidirectional effect. It possesses antiapoptotic, anti-inflammatory, and antioxidative properties that may be beneficial in the treatment of corneal injuries. Moreover, KYNA has been used successfully to improve the healing outcome of skin wounds.

Tryptophan as a Safe Compound in Topical Ophthalmic Medications: and Studies.

Background: To evaluate the effects of tryptophan (TRP) on normal human corneal and conjunctival epithelium in vitro and the re-epithelization of corneal erosion in rabbits.

Materials And Methods: Corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) cultures were used.

Examination of Kynurenine Toxicity on Corneal and Conjunctival Epithelium: In vitro and in vivo Studies.

Kynurenine (KYN) is a metabolite of tryptophan, proposed for the treatment of corneal diseases. Our goal was to evaluate the effects of KYN on normal human corneal and conjunctival epithelial cells in vitro and the re-epithelization of corneal erosion in rabbits. In our study, we used corneal (10.

Osteochondral Repair Using Porous Three-dimensional Nanocomposite Scaffolds in a Rabbit Model.

Aim: To evaluate the utility of a novel nanocomposite biomaterial consisting of poly-L/D-lactide, and hydroxyapatite bioceramics, enriched with sodium alginate in articular cartilage defect treatment.

Materials And Methods: The biomaterial was prepared using the method of solvent casting and particle leaching. The study was conducted on 20 New Zealand White rabbits.

A new model of detrusor overactivity in conscious rats induced by retinyl acetate instillation.

Introduction: A credible animal overactive bladder model used in basic research is an indispensable harbinger of safe and ethical clinical trials on human subjects. Our objective was to develop a new animal model of a hyperactive bladder that will be void of inflammatory urothelium lesions and display significant sensitivity to muscarinic receptor antagonists.

Methods: To examine the influence of 0.

Investigations of ultrastructure of damaged and regenerated skeletal muscle fibers.

Our investigations concerned the head of the parietal part of quadriceps femoris, and we based our investigation on observations of the ultrastructure of muscle fibers using an electron microscope. We observed tissue samples taken from patients (10 men) 25-35 years old, who had old damage of knee joint ligament (after about 6 week's immobilization). In the first group, segments of tissue of parietal head of quadriceps femoris were taken inter-operationally from patients in whom there was found old damage of knee joint ligament.

Histological and ultrastructural changes in cross-striation muscle cells, under the influence of atorvastatin-reductase HMG-CoA inhibitor.

The investigation was carried out on 15 Wistar rats--males weighing about 200 mg each. The animals were divided into three groups: one control group and two experimental groups, with five animals in each. In experimental group I the animals received emulsion of Atorvastatin in distilled water at the therapeutical dose of 80 mg/kg of body mass, by stomach tube for 6 weeks.

Ultrastructure of kidney renal proximal convoluted tubules of experimental animals after Cladribine (2-CdA) administration.

The proximal convoluted tubules of the kidney of white Wistar rats were examined. The animals were given Cladribine (2-CdA) subcutaneously at dosages of 0.07 mg/kg b.

Ultra-structure of the oral cavity epithelial cells in white rat after experimental application of Naran S preparation.

The effectiveness of the natural drug--Naran S was studied in the therapy of the topical inflammation of the oral cavity mucous membrane. The experiment was conducted on male Wistar rats that were subjected to induction of inflammatory condition by injecting Freund adjuvant and rinsing oral cavity with the infusion of the drug for the period of 28 days. The analysis of the ultra structure of the epithelium cells was conducted with the use of transmission electron microscope.

Experimental study of treatment effectiveness of the natural drug--Naran S in inflammatory conditions of the oral cavity in animals.

The aim of the study was the evaluation of the action of a new natural drug--Naran S, elaborated in the Department of Inorganic Chemistry of the Medical University of Lublin (patent registration P.332066) in the treatment of inflammatory conditions of the oral cavity mucous membrane. The experiment was conducted on male Wistar rats.

Histological changes of white rat kidney after experimental administration of ethanol and cephalexin.

The experiment was carried out on Wistar rat males weighing about 200 g. Animals from the control group received water and standard granulated fodder ad libitum. Animals from control group II received 20% ethanol ad libitum instead of water.

[Evaluation of the morphology of the human placental cotyledon following dual in vitro perfusion in variable magnetic field].

Unlabelled: Objectives and the aim of the study was electron-microscopy morphological estimation of the human placental cotyledon after 180 minutes of dual closed perfusion in vitro.

Materials And Methods: In the experimental group the cotyledons were exposed to variable magnetic field of 2 mT magnetic induction and 50 Hz frequency. The control group K (10 perfusions) was not subjected to magnetic field while the experimental group B (10 perfusions) was influenced by magnetic field.

Molecular diagnostics of promyelocytic leukaemia.

Acute promyelocytic leukaemia (APL) is characterised by proliferation of abnormal promyelocytes. The reciprocal translocation between the long arms of chromosomes 15 and 17, and the fusion between the retinoic acid receptor (RARa) gene, and PML gene, is unique to APL. Because of unsuccessful cytogenetic analysis of conventional G-banding technique (mitoses were not observed), we diagnosed three non-treatment patients with APL by following molecular methods: reverse transcription-polymerase chain reaction (RT-PCR), fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH).

Ultrastructural changes of pancreas of white rats after experimental administration of ethanol and cephalexin.

The experiment was carried out on Wistar rat males weighting about 200 g. Animals from experimental group I received 20% ethanol for drinking (ad libitum), animals from experimental group II--cephalexin in the dose of 42 mg/24 h, animals from experimental group III--cephalexin and ethanol in the mentioned doses. After 10 days the animals were decapitated and pancreases were collected for ultrastructural examinations in electron microscope.

Histological examination of the Loeventhal gland after experimental administration of Metizol.

The Loeventhal gland of the white Wistar rats was examined. The animals were given Metizol for 21 days and 42 days at the dose of 1 mg/kg b.m.

Histological examination of the submandibular gland following experimental administration of Metizol.

The submandibular gland of the white Wistar rats was examined. The animals were given Metizol for 21 days and 42 days at the dose of 1 mg/kg b.m.

Ultrastructural changes of ciliary epithelial nucleus in experimental diabetes--an animal model.

The aim of this study was to observe in electron microscope the New Zealand rabbits ciliary epithelial nucleus structure changes, depending on the duration of experimental diabetes mellitus. The animals were administered alloxan in the form of 10% solution in 0.9% NaCl, at the dose of 100 mg/kg b.

Ultrastructural changes in the receptor parts of retinal rods in experimental alloxan-induced diabetes in rabbits.

Mature rabbits were administered a single dose of alloxan at the dose 100 mg/kg b.m. After 3 and 6 weeks and after 3 and 6 months, the samples of the retina were taken from the areas immediate to the papilla of the optic nerve.

Ultrastructural changes in the syncytiotrophoblast in some types of pathological pregnancy.

The studies were performed on 12 placentas from pathological pregnancies (gestosis, cholestasis, type A diabetes pregnancies, premature outflow of amniotic fluids) and 3 placentas from normal pregnancies. Ultrathin sections were stained by the Reynold's method and evaluated under the electron microscope BS-500 (Tesla). The changes found in syncytiotrophoblast were the following: the increase or the decrease in its thickness, increase in basal membrane thickness, decrease in the number or lack of pinocytic vesicles and superficial microvilli, damage of mitochondria.

Histological examinations of the influence of 6-hydroxydopamine (6-OH-DA) upon the ileum of the guinea pig.

Examinations of the influence of 6-OH-DA (6-hydroxydopamine) upon the structure of nerve cells and their axons have been conducted by many authors and in various aspects (1, 2, 8, 10). They have concerned histochemistry, fluorescence and ultrastructure of neurons and have shown that the substance evokes various changes in the perikaryons and axons of adrenergic neurons. The wall of the small intestine is innervated by external autonomyous nerves and fibres of the intramural autonomyous plexuses.

The influence of encortone on ultrastructural changes in the parotid cells of female white rats of Wistar race.

It is generally known that glucocorticoids exert an essential influence on digestive tract physiology (2). Many investigations carried out refer to the changes observed in the pancreas under different experimental conditions (4, 5, 6, 7, 8). The investigations of the influence of glucocorticoids on salivary glands and mainly on a parotid gland are very general.