A functional gene encoding carnitine palmitoyltransferase 1 and its transcriptional and kinetic regulation during fasting in large yellow croaker.

Carnitine palmitoyltransferase 1 (CPT1) plays an essential role in maintaining energy supply via fatty acid oxidation, especially under fasting. In this study, the complete cDNA sequence of cpt1a was cloned from liver of large yellow croaker (Larimichthys crocea), with an open reading frame of 2319 bp encoding a protein of 772 amino acids. Bioinformatics analysis predicted the presence of conserved functional motifs and amino acid residues.

Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure.

In the study, zebrafish were exposed to 0 and 200 μg/L Zn for 8 weeks, and then both groups were transferred to water including 0, 100, and 200 μg/L Cd for 4 days, respectively. Acute Cd exposure caused negative effects on stress defense, immune, and metal transport systems by increasing lipid peroxidation, iNOS activity and mRNA levels of il-6 and inos, and decreasing Cu/Zn-SOD and HSP70 levels, and mRNA levels of sod1, cat, hsp70, p65, mtf-1, znt5, zip7, atp7a, and atp7b. Lipid peroxidation was significantly reduced by Zn pre-exposure under Cd exposure, which may be explained by the enhanced stress defense capacity and the weaken inflammatory response.

The lagged effects of environmentally relevant zinc on non-specific immunity in zebrafish.

Responses to zinc (Zn) during exposure have well studied but the effects after the exposure are commonly neglected. In the study, non-specific immune response to zinc in blood and spleen of zebrafish was evaluated after exposure. At first, fish were subjected to 0 (control) and 200 μg/L zinc (Zn) for 6 weeks.