Secondary bloodstream infection in critically ill patients with COVID-19.

Objective: Secondary infection, especially bloodstream infection, is an important cause of death in critically ill patients with COVID-19. We aimed to describe secondary bloodstream infection (SBI) in critically ill adults with COVID-19 in the intensive care unit (ICU) and to explore risk factors related to SBI.

Methods: We reviewed all SBI cases among critically ill patients with COVID-19 from 12 February 2020 to 24 March 2020 in the COVID-19 ICU of Jingmen First People's Hospital.

Huygens-Fresnel Picture for High Harmonic Generation in Solids.

High harmonic generation (HHG) is usually described by the laser-induced recollision of particlelike electrons, which lies at the heart of attosecond physics and also inspires numerous attosecond spectroscopic methods. Here, we demonstrate that the wavelike behavior of electrons plays an important role in solid HHG. By taking an analogy to the Huygens-Fresnel principle, an electron wave perspective on solid HHG is proposed by using the wavelet stationary-phase method.

Wave-packet behaviors of the defocusing nonlinear Schrödinger equation based on the modified physics-informed neural networks.

Investigated in this paper is the defocusing nonlinear Schrödinger (NLS) equation, which is used for describing the wave-packet dynamics in certain weakly nonlinear media. With the physics-informed neural networks (PINNs), we modify the corresponding loss function in the existing literature and obtain two types of dark solitons, type-I and type-II solitons. It is demonstrated that the modified loss function presents higher-precision wave-packet behaviors based on fewer initial and boundary data.

Inhibition of the PLK1-Coupled Cell Cycle Machinery Overcomes Resistance to Oxaliplatin in Colorectal Cancer.

Dysregulation of the cell cycle machinery leads to genomic instability and is a hallmark of cancer associated with chemoresistance and poor prognosis in colorectal cancer (CRC). Identifying and targeting aberrant cell cycle machinery is expected to improve current therapies for CRC patients. Here,upregulated polo-like kinase 1 (PLK1) signaling, accompanied by deregulation of cell cycle-related pathways in CRC is identified.

Prevalence and Characteristics of Ceftriaxone-Resistant in Children's Hospital in Hangzhou, China.

The non-Typhi (NTS) infection is critical to children's health, and the ceftriaxone is the important empirical treatment choice. With the increase resistance rate of ceftriaxone in , the molecular epidemiology and resistance mechanism of ceftriaxone-resistant needs to be studied. From July 2019 to July 2020, a total of 205 NTS isolates were collected, 195 of which (95.

Split stoma with delayed anastomosis may be preferred for 2-stage surgical resection in high-risk patients with Crohn's disease.

Background: Fecal diversion after bowel resection is a safe and effective procedure in high-risk patients with Crohn's disease, but the better approach between primary anastomosis with protective stoma and split stoma with delayed anastomosis has not yet been investigated. This study aimed to compare the outcomes of these approaches in high-risk patients with Crohn's disease.

Methods: A retrospective investigation on consecutive high-risk patients with Crohn's disease was conducted at a tertiary referral hospital from August 2009 to March 2019.

Chemical grafting fluoropolymer on cellulose nanocrystals and its rheological modification to perfluoropolyether oil.

Cellulose nanocrystal (CNC) is the sustainable nanoparticle derived from natural cellulose, widely used as the functional additive to tailor the properties and performances of diverse matters. This study attempted the application of CNC as the rheological modifier in fluorocarbon lubricating oil (perfluoropolyether, PFPE). To solve the incompatibility between cellulose and base oil, fluoropolymer was covalently grafted onto the surface of nanocrystals by living radical polymerization.

Microbiota in mesenteric adipose tissue from Crohn's disease promote colitis in mice.

Background: Mesenteric adipose tissue (mAT) hyperplasia, known as creeping fat is a pathologic characteristic of Crohn's disease (CD). The reserve of creeping fat in surgery is associated with poor prognosis of CD patients, but the mechanism remains unknown.

Methods: Mesenteric microbiome, metabolome, and host transcriptome were characterized using a cohort of 48 patients with CD and 16 non-CD controls.

Comprehensive Analysis of the Expression and Prognosis for MMPs in Human Colorectal Cancer.

Background: Previous study implicated that genes of matrix metalloproteinase (MMP) family play an important role in tumor invasion, neoangiogenesis, and metastasis. However, the diverse expression patterns and prognostic values of 24 MMPs in colorectal cancer are yet to be analyzed.

Methods: In this study, by integrating public database and our data, we first investigated the expression levels and protein levels of MMPs in patients with colorectal cancer.

Zipper head mechanism of telomere synthesis by human telomerase.

Telomerase, a multi-subunit ribonucleoprotein complex, is a unique reverse transcriptase that catalyzes the processive addition of a repeat sequence to extend the telomere end using a short fragment of its own RNA component as the template. Despite recent structural characterizations of human and Tetrahymena telomerase, it is still a mystery how telomerase repeatedly uses its RNA template to synthesize telomeric DNA. Here, we report the cryo-EM structure of human telomerase holoenzyme bound with telomeric DNA at resolutions of 3.

Enhancing Photocatalytic Hydrogen Production via the Construction of Robust Multivariate Ti-MOF/COF Composites.

Titanium metal-organic frameworks (Ti-MOFs), as an appealing type of artificial photocatalyst, have shown great potential in the field of solar energy conversion due to their well-studied photoredox activity (similar to TiO ) and good optical responsiveness of linkers, which serve as the antenna to absorb visible-light. Although much effort has been dedicated to developing Ti-MOFs with high photocatalytic activity, their solar energy conversion performances are still poor. Herein, we have implemented a covalent-integration strategy to construct a series of multivariate Ti-MOF/COF hybrid materials PdTCPP⊂PCN-415(NH )/TpPa (composites 1, 2, and 3), featuring excellent visible-light utilization, a suitable band gap, and high surface area for photocatalytic H production.

Endoscopic Stricturotomy for Patients With Postoperative Benign Anastomotic Stricture for Colorectal Cancer.

Background: Postoperative benign anastomotic stricture is associated with colorectal anastomosis following surgery for colorectal cancer. Endoscopic stricturotomy is a novel technique that has been demonstrated to be safe and effective for the treatment of colorectal anastomotic stricture in several case reports and series.

Objective: We designed this study to investigate the efficacy of endoscopic stricturotomy for postoperative benign anastomotic stricture in patients for colorectal cancer.

Emergence of Ceftazidime/Avibactam and Tigecycline Resistance in Carbapenem-Resistant Due to In-Host Microevolution.

can cause both hospital- and community-acquired clinical infections. Last-line antibiotics against carbapenem-resistant (CRKP), such as ceftazidime/avibactam (CZA) and tigecycline (TGC), remain limited as treatment choices. This study aimed to investigate the mechanisms by which CRKP acquires CZA and TGC resistance under β-lactam antibiotic and TGC exposure.

Hydrophobic and thermal-insulating aerogels based on rigid cellulose nanocrystal and elastic rubber.

Inspired from the ancient China philosophy of "coupling hardness with softness", we attempted the combination of rigid cellulose nanocrystals (CNC) and elastic rubbers to solve the limitations of structural brittleness and water sensitivity of CNC-based aerogels. Three rubber chains with the different chemical structures (silicon rubber, 1,2-polybutadiene, styreneic block copolymer) were covalently bonded on the CNC porous skeleton based on thiol-ene click chemistry, to fabricate the CNC/rubber composite aerogels. With the introduction of moderate loading levels of rubber, the composites aerogels exhibited low density and shrinkage, high porosity and specific surface area and improved mechanical performance.

Cancer-associated fibroblasts impact the clinical outcome and treatment response in colorectal cancer via immune system modulation: a comprehensive genome-wide analysis.

Background: Cancer-associated fibroblasts (CAFs) in the tumour microenvironment are associated with poor prognosis and chemoresistance in multiple solid tumours. However, there is a lack of universal measures of CAFs in colorectal cancer (CRC). The aim of this study was to assess the utility of a fibroblast-related gene signature (FRGS) for predicting patient outcomes and reveal its relevant mechanism.

Donor-specific antibodies, glomerulitis, and human leukocyte antigen B eplet mismatch are risk factors for peritubular capillary C4d deposition in renal allografts.

Background: The complement system plays an important role in the immune response to transplantation, and the diagnostic significance of peritubular capillary (PTC) C4d deposition (C4d+) in grafts is controversial. The study aimed to fully investigate the risk factors for PTC C4d+ and analyze its significance in biopsy pathology of kidney transplantation.

Methods: This retrospective study included 124 cases of kidney transplant with graft biopsy and donor-specific antibody (DSA) testing from January 2017 to December 2019 in a single center.

Identification of two novel poleroviruses and the occurrence of Tobacco bushy top disease causal agents in natural plants.

Tobacco bushy top disease (TBTD) is a devastating tobacco disease in the southwestern region of China. TBTD in the Yunnan Province is often caused by co-infections of several plant viruses: tobacco bushy top virus (TBTV), tobacco vein distorting virus (TVDV), tobacco bushy top virus satellite RNA (TBTVsatRNA) and tobacco vein distorting virus-associated RNA (TVDVaRNA). Through this study, two new poleroviruses were identified in two TBTD symptomatic tobacco plants and these two novel viruses are tentatively named as tobacco polerovirus 1 (TPV1) and tobacco polerovirus 2 (TPV2), respectively.

Evaluation of ultrastructural alterations of glomerular basement membrane and podocytes in glomeruli by low-vacuum scanning electron microscopy.

Background: Low-vacuum scanning electron microscopy (LV-SEM) is applied to diagnostic renal pathology.

Methods: To demonstrate the usefulness of LV-SEM and to clarify the optimal conditions of pathology samples, we investigated the alterations of glomerular basement membrane (GBM) and podocytes in control and experimental active Heymann nephritis (AHN) rats by LV-SEM.

Results: On week 15 following induction of AHN, spike formation on GBM with diffuse deposition of IgG and C3 developed.

PERK13 modulates phosphate deficiency-induced root hair elongation in Arabidopsis.

Phosphate starvation (-Pi)-induced root hair is crucial for enhancing plants' Pi absorption. Proline-rich extensin-like receptor kinase 13 (PERK13) is transcriptionally induced by -Pi and co-expressed with genes associated with root hair growth. However, how PERK13 participates in -Pi-induced root hair growth remains unclear.

SIRVD-DL: A COVID-19 deep learning prediction model based on time-dependent SIRVD.

COVID-19 is one of the biggest challenges that human beings have faced recently. Many researchers have proposed different prediction methods for establishing a virus transmission model and predicting the trend of COVID-19. Among them, the methods based on artificial intelligence are currently the most interesting and widely used.

Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.

Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation. To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions. Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO/chitin hydrogel exhibited loose macropores and mesopores.

Gut Microbiome Alterations in COVID-19.

Since the outset of the coronavirus disease 2019 (COVID-19) pandemic, the gut microbiome in COVID-19 has garnered substantial interest, given its significant roles in human health and pathophysiology. Accumulating evidence is unveiling that the gut microbiome is broadly altered in COVID-19, including the bacterial microbiome, mycobiome, and virome. Overall, the gut microbial ecological network is significantly weakened and becomes sparse in patients with COVID-19, together with a decrease in gut microbiome diversity.