Testacosides A-D, glycoglycerolipids produced by Microbacterium testaceum isolated from Tedania brasiliensis.

Marine bacteria living in association with marine sponges have proven to be a reliable source of biologically active secondary metabolites. However, no studies have yet reported natural products from Microbacterium testaceum spp. We herein report the isolation of a M.

Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis.

Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products.

Isolation of Water-Soluble Metabolites from Marine Invertebrates and Microorganisms.

The isolation of pure organic compounds from biological sources, reaction media, or other complex molecular matrixes is the first step to overcome before further biological and chemical investigations. While the isolation of chemicals soluble in organic solvents is commonly accomplished, the isolation of water-soluble organic compounds is less often addressed. We present here a simple method for the isolation of water-soluble organic compounds, using adsorptive macroporous resins and reversed-phase chromatography.

Approaches for the isolation and identification of hydrophilic, light-sensitive, volatile and minor natural products.

Covering: up to 2019The discovery of new bioactive natural products gained momentum during the last few decades, resulting from instrumentation advances, from the expansion of genome mining and regulation, as well as by exploration of untapped biological sources. However, water-soluble, volatile, minor and photosensitive natural products are yet poorly known. This review discusses the literature reporting the isolation strategies for some of these metabolites.