Cryopreservation of human amniotic membrane for ocular surface reconstruction: a comparison between protocols.

Purpose: To compare the effects on adhesive and structural properties of newer preservation conditions to those obtained with an established, standardized protocol (dimethyl sulfoxide at -180 °C). In attempt to simplify and enhance the safety of the procedure, we tested dextran-based freezing medium and a dry condition (no medium) at temperatures of -80 °C.

Methods: Five patches of human amniotic membrane were obtained from three different donors.

Culture of corneal endothelial cells obtained by descemetorhexis of corneas with Fuchs endothelial corneal dystrophy.

Currently, endothelial keratoplasty is the gold standard for the surgical treatment of Fuchs endothelial corneal dystrophy (FECD). Despite the remarkable success in terms of surgical outcomes, a shortage of corneal donor tissue poses a limitation to performing endothelial keratoplasty in many parts of the world. Cell therapy is a potential alternative strategy to keratoplasty and is currently under investigation.

A new standardized immunofluorescence method for potency quantification (SMPQ) of human conjunctival cell cultures.

The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy clinical studies when fewer cells are available to perform the quality controls. To achieve this purpose, a reference standard was investigated and the expression levels of ΔNp63α (considered as a marker of conjunctival stem cells) was correlated to cell size.

Correction to: Establishing a Reproducible Murine Animal Model of Single Anastomosis Duodenoileal Bypass with Sleeve Gastrectomy (SADl-S).

The first and last names of the authors were inverted in the original article. They are displayed correctly here.

Cognitive impairment in late-onset depression. Limited to a decrement in information processing resources?

Background: While cognitive dysfunction in late-onset depression (LOD) is common, the nature and determinants of this impairment are heterogeneous. It has been suggested that neuropsychological decrements in LOD patients might result from a deficit in processing resources. In order to address this issue, we analyzed processing resources in LOD to see if their decrease explains higher-level cognition (episodic memory and naming capacity) deficits.

Inducing emotion by unilateral contraction of hand muscles.

Following 4 unilateral contractions of hand muscles, subjects told stories about pictures from the T.A.T.

Unilateral contraction of facial muscles do effect emotions: a "failed replication's" failure to perform a replication.

Kop, Merckelbach and Muris (1991) reported a failure to replicate Schiff and Lamon's (1989) finding that unilateral muscle contractions induce emotions and influence cognitions in a manner which reflect those emotions. However, the procedures they used were different from the original experiment in substantive ways. These differences are described and it is explained how they account for the failure to replicate.

Unilateral Facial Contractions Produce Mood Effects on Social Cognitive Judgements.

This study demonstrates that left and right unilateral facial contractions have similar effects on the expression of ethnic stereotypes as do negative and positive moods induced by more conventional means. Subjects who con tracted the left side of their face (negative mood inducer) were more likely to express negative stereotypes of ethnic groups than were subjects who contracted the right side of their face (positive mood inducer). This parallels previous findings obtained using two standard mood inductions: the Veltens mood induction procedure; and a musical mood induction procedure.

Examination of Raynaud's phenomenon in traumatic vasospastic disorders.

Certain types of occupation-related vibrations can cause traumatic vasospastic disorders. Findings from clinical examination are often normal at the office consultation. Measurements of finger blood flow using plethysmography while the local ambient temperature is changed can give important information about the severity of traumatic vasospastic disorder and the concomitant disability.

Inducing emotion by unilateral contraction of facial muscles: a new look at hemispheric specialization and the experience of emotion.

Subjects who maintained voluntary contractions of the left facial muscles experienced sadness. Right facial contractions resulted in a more positive but difficult to characterize experience. These contractions had similar effects on the affective tone of stories told about an ambiguous picture.

Conceptual transfer insimple insight problems.

Three experiments explored the conditions under which information presented in the first part of an experiment facilitates the subsequent solving of simple insight problems. We argue that previous unsuccessful attempts to obtain such facilitation are attributable to the experimenters' failure to present this information in a form that induces the conceptual operations needed to solve the problem. Substantial facilitation is obtained if the information is presented in a form that induces a few seconds of puzzlement and then a clue is presented that leads to an appropriate reconception; if identical information is presented without such a period of puzzlement and reconception, no facilitation is observed.

3-Hydroxyproline content of normal urine.

Values for total 3-hydroxyproline and 4-hydroxyproline were obtained from 24-h urine specimens of 18 healthy human subjects of both sexes, whose ages ranged from the first to the sixth decade in age. Urinary 3-hydroxyproline levels, not earlier described to our knowledge, were determined by an isotope-dilution method requiring considerable purification and utilizing the amino acid analyzer for final measurement. 3-Hydroxyproline averaged 3% of the corresponding 4-hydroxyproline in individual urine samples.

Prolyl hydroxylase of earthworms. Substrate specificity of an enzyme from the subcuticular epithelium.

A relatively crude enzyme preparation derived from the subcuticular epithelium of earthworms catalyzed the formation of 4-hydroxyproline from prolyl residues in unhydroxylated natural collagens and in several synthetic collagen-like polypeptides. The specificity of hydroxylation differed from that of all vertebrate polyl hydroxylases in that (Gly-Pro-Ala)n was a much better substrate than (Gly-Ala-Pro)n. In contrast, however, only the so-called Y position proline (Gly-X-Y) was hydroxylated in Gly-Pro-Pro sequences derived either from natural collagen or from synthetic polypeptides; specificity of hydroxylation for the latter sequence is identical with that of the vertebrate enzymes.

Sequence position of 3-hydroxyproline in basement membrane collagen. Isolation of glycyl-3-hydroxyprolyl-4-hydroxyproline from swine kidney.

The position of 3-hydroxyproline was investigated in the triplet sequences of peptides released by collagenase digestion of a collagen preparation from kidney cortex. Composition of the collagen preparation indicated that it was largely or wholly of basement membrane origin. 3-Hydroxyproline was detected in only one sequence, the tripeptide, glycyl-3-hydroxyprolyl-4-hydroxyproline, which accounted for a major fraction of the total 3-hydroxyproline obtained in the peptides released by collagenase.