Responds to the Central Metabolite Pyruvate To Regulate Virulence.

is a versatile bacterial pathogen that can cause significant disease burden and mortality. Like other pathogens, must adapt to its environment to produce virulence factors to survive the immune responses evoked by infection. Despite the importance of environmental signals for pathogenicity, only a limited number of these signals have been investigated in detail for their ability to modulate virulence.

Staphylococcus aureus pathogenesis in diverse host environments.

Staphylococcus aureus is an eminent human pathogen that can colonize the human host and cause severe life-threatening illnesses. This bacterium can reside in and infect a wide range of host tissues, ranging from superficial surfaces like the skin to deeper tissues such as in the gastrointestinal tract, heart and bones. Due to its multifaceted lifestyle, S.

Organic small hairpin RNAs (OshR): a do-it-yourself platform for transgene-based gene silencing.

The RNA interference (RNAi) pathway in animal cells can be harnessed to silence gene expression with artificial small interfering RNAs (siRNAs) or transgenes that express small hairpin RNAs (shRNAs). The transgene-expressing shRNA approach has been adapted into large-scale resources for genome-wide loss-of-function screens, whereas focused studies on a narrow set of genes can be achieved by using individual shRNA constructs from these resources. Although current shRNA repositories generally work, they might fail in certain situations and therefore necessitate other alternatives.