Induction of apoptosis by emestrin from the plant endophytic fungus ATCC 38163 in Huh-7 human hepatocellular carcinoma cells.

This research aimed to investigate the anticancer properties of emestrin, a major constituent of ATCC 38163 through the induction of apoptosis in Huh-7 human hepatocellular carcinoma cells. In this study, this fungus was isolated from the fresh leaves of (Cham. & Schltdl.

Flavonoid constituents and protective efficacy of (Blanco) leaves ethanolic extract on thioacetamide-induced liver injury rats.

Oxidative stress leads to deleterious processes in the liver that resulted in liver diseases. To evaluate antioxidant activity and hepatoprotective potential of ethanolic leaves extract of against hepatic dysfunction induced by thioacetamide (TAA). Flavonoid constituents were isolated from the ethanol extract by chromatographic techniques and identified by the spectroscopic analyses.

Halo-phenolic metabolites and their in vitro antioxidant and cytotoxic activities from the Red Sea alga .

From the green alga two new naturally halo-benzaldehyde derivatives were isolated by various chromatographic methods along with 10 known metabolites of bromophenols, sulfonoglycolipid, and steroids. Based on the 1D and 2D NMR spectra as well as on MS data, the structures of the new compounds were identified as 5-bromo-2-(3-bromo-4-hydroxybenzyl)-3,4-dihydroxybenzaldehyde named avrainvilleal (), and 3-iodo-4-hydroxy-benzaldehyde (). Using SRB assay, both compounds showed mild and weak cytotoxic activity against HeLa and MCF-7 cancer cell lines, compared to the good activity of their extract (IC values 3.

Rotenoid and isoflavone metabolites from an antioxidant seed extract of (roxb.) thoth.

A new rotenoid named 12--methylrotenolol along with five known rotenoid and isoflavone metabolites were isolated from the seeds of , collected from Egypt. The structures of these compounds were identified by physical and spectroscopic data measurements ([α], UV, 1D- and 2D-NMR and MS). The methanol extract of the seeds exhibited strong antioxidant activity with IC value 0.

inhibition of Hepatitis C virus protease and antioxidant by flavonoid glycosides from the Saudi costal plant .

A new flavonol triglycoside, rhamnazin 3-O-2-rhamnorutinoside or rhamnazin 3--(2″,6″--α-di-rhamnosyl)-β-glucoside () was isolated along with known flavonols, rhamnazin 3--rutinoside (), rhamnazin 3--(6″--α-rhamnosyl)-β-galactoside (), isorhamnetin 3--(6″--α-rhamnosyl)-β-galactoside (), isorhamnetin 3--(2″,6″--α-di-rhamnosyl)-β-galactoside (), and isorhamnetin (), and allantoin () from the aqueous methanol extract of leaves. Spectral analyses (UV, MS, and NMR) and acid hydrolysis were used to determine the structures. These compounds in this study except were reported for the first time from the genus .

Sesquiterpenes from the Saudi Red Sea: Litophyton arboreum with their cytotoxic and antimicrobial activities.

A new pseudoguaiane-type sesquiterpene named litopharbol (1) was isolated from the methanolic extract of the Red Sea soft coral Litophyton arboreum, along with known sesquiterpenoids alismol (2), alismorientol B (3), teuhetenone A (4), and calamusin I (5); steroid, 24-methyl-cholesta-5,24(28)-diene-3β-ol (6), alkyl glyceryl ether, chimyl alcohol (7); sphingolipid, erythro-N-dodecanoyl-docosasphinga-(4E,8E)-dienine (8); and nitrogenous bases, thymine (9) and thymidine (10). The structures were determined on the basis of nuclear magnetic resonance (NMR) spectroscopic (1D and 2D NMR data including heteronuclear single quantum coherence spectroscopy, heteronuclear multiple-bond correlation spectroscopy, and nuclear Overhauser effect spectroscopy) and mass spectrometric analyses. Compounds 1-5 were explored for antimicrobial activity and cancer cell line sensitivity tests.

Cytotoxic activity of alkyl benzoate and fatty acids from the red sea sponge Hyrtios erectus.

The chemical investigation of the methylene chloride fraction of marine sponge Hyrtios erectus led to the isolation of the known oxysterol (2) along with a new alkyl benzoate compound identified by spectroscopic methods (NMR and MS) as 4'-methylheptyl benzoate (1), whilst the n-butanol fraction afforded the known indole 3-carbaldehyde and β-carboline derivatives. Moreover, the hexane fraction was analysed by GC-MS for their fatty acids (FAs). A total of 17 FAs with chain lengths between 14 and 25 carbons were identified.

Thalassiolin D: a new flavone O-glucoside Sulphate from the seagrass Thalassia hemprichii.

Thalassiolin D, a new flavone O-glucoside sulphate along with three flavonoids, two steroids, p-hydroxybenzoic acid, 4,4'-dihydroxybenzophenone and nitrogen compound, octopamine were isolated from the seagrass Thalassia hemprichii, collected from the Saudi Red Sea coast. By extensive spectroscopic analysis including 1D and 2D NMR and MS data, the structure of the new compound was elucidated as diosmetin 7-O-β-glucosyl-2″-sulphate. The new compound displayed moderately in vitro antiviral HCV protease activity with IC value 16 μM.

Different Culture Metabolites of the Red Sea Fungus Fusarium equiseti Optimize the Inhibition of Hepatitis C Virus NS3/4A Protease (HCV PR).

The endophytic fungus was isolated from the brown alga , collected from the Red Sea. The fungus was identified by its morphology and 18S rDNA. Cultivation of this fungal strain in biomalt-peptone medium led to isolation of 12 known metabolites of diketopeprazines and anthraquinones.

Chemical Composition and Antifungal Activity of Ocimum basilicum L. Essential Oil.

Background: The leaves of Ocimum basilicum L. (basil) are used in traditional cuisine as spices; its essential oil has found a wide application in perfumery, dental products as well as antifungal agents.

Aim: To assess the chemical composition as well as the in vitro antifungal activity of O.

A new flavonoid C-glycoside from Solanum elaeagnifolium with hepatoprotective and curative activities against paracetamol-induced liver injury in mice.

A new flavonoid C-glycoside, kaempferol 8-C-beta-galactoside, along with twelve known glycosidic flavonoids was isolated from the aqueous methanolic extract of Solanum elaeagnifolium Cav. (Solanaceae), by conventional chromatographic methods; their structure elucidation was achieved using UV, ESI-MS, and NMR spectral analyses. Groups of six mice were administered S.

Digalacturonide flavones from Egyptian Lantana camara flowers with in vitro antioxidant and in vivo hepatoprotective activities.

A new digalacturonide flavone, luteolin 7-O-beta-galacturonyl-(2 --> 1)-O-beta-galacturonide (1), was isolated along with nine known flavone glycosides from the aqueous methanolic extract of Lantana camara (L.) flowers. Their structures were determined on the basis of the spectral data.

Effect of Ruta graveolens L. and Euphorbia peplus L. anti-inflammatory extracts on nutritional status of rats and the safety of their use.

A significant increase in body weight with remarkable increase in total food intake and significant increase in protein efficiency ratio were observed following oral administration of R. graveolens ether extract (500 mg/kg body wt) to growing rats for 3 weeks. Serum albumin was significantly decreased after administration of declofenac (15 mg/kg body wt).