Improving porcine in vitro blastocyst development using fetal bovine serum, amino acids, and insulin-transferrin-selenium.

In vitro production of porcine-hatched blastocysts is important for various applications. However, the mechanobiology of blastocoel expansion and hatching remains poorly understood. Our study aimed to efficiently produce hatched blastocysts and investigate the hatching mechanics of late-stage porcine embryos using time-lapse assessment.

Melatonin and cyclic adenosine monophosphate enhance the meiotic and developmental competence of porcine oocytes from early antral follicles during in vitro growth and pre-maturation culture.

Melatonin has been studied for its ability to improve oocyte quality and modulate cyclic adenosine monophosphate (cAMP) production. However, the effects of melatonin on the in vitro growth (IVG) of oocyte-cumulus-granulosa complexes (OCGCs) derived from early antral follicles (EAFs) have not been fully investigated. This study aimed to examine the effects of melatonin during IVG on the developmental competence and blastocyst quality of porcine oocytes isolated from EAFs.

Agarose-based 3D culture improved the developmental competence of oocyte-granulosa complex isolated from porcine preantral follicle.

Various models have been established to culture whole follicles of the Preantral stage; however, the process remains inefficient and is an ongoing challenge formation. It is reported that oocyte-cumulus-granulosa complexes (OCGCs) isolated from Early Antral follicles (EAFs) undergo in vitro growth (IVG) and acquire meiotic competence in some animals. However, IVG for the oocyte-granulosa complexes (OGCs) from Preantral Follicles (PAFs) has not been firmly established.