Development of an aptasensor for highly sensitive detection of cardiac troponin I using cobalt-nickel metal-organic framework (CoNi-MOF).

Objective And Rationale: This study aimed to develop a highly sensitive and selective single-stranded DNA (ssDNA) aptamer targeting cardiac troponin I (cTnI), a crucial biomarker for acute myocardial infarction (AMI). The objective was to fabricate a novel aptamer electrochemical sensor using a composite material of cobalt-nickel metal-organic framework (CoNi-MOF) on screen-printed carbon electrodes (SPCE), leveraging the composite's large surface area and excellent electrical conductivity alongside the aptamer's high affinity for cTnI.

Methods: The aptamer electrochemical sensor was fabricated using the CoNi-MOF composite on SPCE and characterized its properties.