Background: subspecies (MAP) is the causative agent of Johne's disease (JD), or paratuberculosis in ruminants has been suspected to be implicated in the pathogenesis of Crohn's disease (CD) in humans with chronic inflammatory intestinal changes. As the hypothesis is now fast being recognized that MAP could possibly be the etiological agent of CD which is found to be excreted in milk of dairy animals subclinically or terminally ill with JD.
Aim: The present study was aimed to detect MAP in milk by polymerase chain reaction (PCR) targeting IS and to describe the excretion pattern of MAP in milk from asymptomatic lactating cows and does with relevance to the public health significance.