Inhibitory Effect of Human Anti-CA I Autoantibodies and Development of Monoclonal Antibody mAb 2B8 Targeting Carbonic Anhydrase I.

Spontaneous tumor regression is a recognized phenomenon across various cancer types. Recent research emphasizes the alterations in autoantibodies against carbonic anhydrase I (CA I) (anti-CA I) levels as potential prognostic markers for various malignancies. Particularly, autoantibodies targeting CA I and II appear to induce cellular damage by inhibiting their respective protein's catalytic functions.

Spontaneous regression of tumours. Possible cross reactivity of autoantibodies against carbonic anhydrase I.

Spontaneous tumour regression in patients after high dose therapy and autologous stem cell transplantation or patients with standard therapy is accompanied with the presence of high titers autoantibodies against carbonic anhydrase I (CA I). The concomitant presence of aplastic anaemia-like syndrome in these patients points to parallel bone marrow suppression during this period. It seems that CA I, an 'obscure' enzyme, does not have any significant physiological role in humans.

Cryoprotective Mechanism of DMSO Induced by the Inhibitory Effect on Eutectic NaCl Crystallization.

Cryopreservation is a critical procedure in autologous hematopoietic stem cell transplantation. Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice. Optimization of the cryopreservation protocol in the past revealed a dramatic loss of cell viability associated with a reduction of the DMSO concentration below 2 vol % in the freezing medium.

On crystallization of water confined in liposomes and cryoprotective action of DMSO.

In this work, the phase behavior of cryoprotective mixtures based on dimethyl sulfoxide (DMSO) mixed with a lipid bilayer consisting of dimyristoyl phosphatidylcholine (DMPC) was studied. This system represented a model of a biological cell and its membrane. The aim of the work was to clarify the origin of the cryoprotective action of low-concentrated mixtures (1-10 vol%) DMSO in water, representing mixtures used in cryopreservation in cell therapy.

Bathyscidius mikati (Udržal, 1995), a new combination from the genus Bathyscia Schiødte, 1848 (Insecta, Coleoptera, Leiodidae, Cholevinae, Leptodirini).

The study of the type material as well as the additional specimens collected in the Rumija Mts., southeastern Montenegro revealed that Bathyscia mikati Udržal, 1995 belongs to the genus Bathyscidius Jeannel, 1910. New combination, Bathyscidius mikati (Udržal, 1995) comb.

Two new species of the genus Blattochaeta Reitter, 1910 (Insecta, Coleoptera, Leiodidae, Cholevinae, Leptodirini) from Montenegro.

Two further new species of the tribe Leptodirini Lacordaire, 1854 inhabit the subterranean world of the Southern Dinarides located in the northern and southeastern mountains of Montenegro: Blattochaeta brankojalzici D. Čeplík, Lakota J. Čeplík, sp.

Bathyscidius basarai, a new species of the tribe Leptodirini Lacordaire, 1854 (Insecta, Coleoptera, Leiodidae, Cholevinae) from Albania.

Bathyscidius basarai D. Čeplík, Lakota J. Čeplík, sp.

Overexpression of CA1 mRNA and the CA I Protein in Tumor Cells Does Not Change the Gene Expression of the ECM Proteins.

In our study, we performed retroviral transduction to overexpress codon-optimized variant of gene encoding human carbonic anhydrase I (optiCA1) in two tumor cell lines PC3 and MDA-MB-231, derived from human prostatic and breast carcinoma respectively. We achieved significantly enhanced and stable overexpression of exogenous optiCA1 gene. The expression of endogenous, wild CA1 gene was found to be normally low (C 28.

Microstructural free volume and dynamics of cryoprotective DMSO-water mixtures at low DMSO concentration.

This work investigates the free-volume properties of the dimethyl sulfoxide (DMSO)-water mixtures by positron annihilation lifetime spectroscopy over a wide temperature range of 20-320 K. The processes of melting and solidification of the water, DMSO and the DMSO-water mixtures at 1.8, 2.

Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells.

We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock-down. The protein composition of the exosomes' cargo was also altered.

Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins.

We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels of mRNA coding CA1 protein in the knock-down mRNA (CA1 siRNA) cells have been measured by RT-PCR and were approximately 5% (PC3) and 20% (MDA-MB-231), respectively, of the level of control (Mock siRNA) used during silencing. In PC3 and MDA-MB-231 cells, the mRNAs for COL1A1 and COL4A4 were up-regulated.

Studies on Noesske with the description of five new species from the Dinarides (Coleoptera: Carabidae: Trechini).

The species belonging to the trechine genus Adriaphaenops Noesske, 1928 are studied. As a result, seven currently known and five newly described species are recognized. Morphological characters of the habitus (especially the shape of head and pronotum) as well as male genitalia are widely used to delimit the species.

Sera of patients with spontaneous tumour regression and elevated anti-CA I autoantibodies change the gene expression of ECM proteins.

Spontaneous tumour regression after high-dose therapy and autologous stem cell transplantation is associated with the aplastic anaemia-like syndrome and the presence of polyclonal autoantibodies against carbonic anhydrase I (CA I). When tumour cells were grown in vitro in the presence of patients' sera positive for anti-CA I autoantibodies, their morphological pattern was altered. These changes were accompanied by modifications in the gene expression profile.

Endogean and cavernicolous Coleoptera of the Balkans. XVII. A new species of the genus Speluncarius Reitter, 1886 (Coleoptera, Carabidae, Harpalinae, Pterostichini) from Croatia.

The genus Speluncarius Reitter, 1886 (Harpalinae, Pterostichini) is made up by anophthalmous or almost blind species. The genus is related to Tapinopterus Schaum, 1858, which contains numerous species from the Balkan-Anatolian region and have more or less developed eyes. Speluncarius is distributed in the Western Palaearctic region; 26 species and one subspecies are known from Italy, the Balkan Peninsula and Turkey.

Treatment of metastatic head and neck cancer with mesenchymal stem cells combined with prodrug gene therapy.

This is a clinical observation of a patient treated for metastatic head and neck cancer with mesenchymal stem cells mediated prodrug gene therapy. The cells were applied intravenously. We did not observe any therapeutic effect.

Synonymy of Reinholdina deelemanorum Giachino & Moravec, 2009 (Coleoptera: Leiodidae: Cholevinae: Leptodirini).

Reinholdina deelemanorum Giachino & Moravec, 2009 is a junior synonym of Parapropus neumanni Müller, 1911. The genus Reinholdina Giachino & Moravec, 2009 is placed in the synonymy with Parapropus Ganglbauer, 1899.

Overlap of epitopes recognized by anti-carbonic anhydrase I IgG in patients with malignancy-related aplastic anemia-like syndrome and in patients with aplastic anemia.

High titers of anti-carbonic anhydrase I (anti-CA I) autoantibodies were detected in the sera of patients with malignancies who developed an aplastic anemia-like (AA-like) syndrome after a high-dose therapy (HDT) and autologous stem cell transplantation (ASCT). It was found, that the presence of these anti-CA I autoantibodies is associated with spontaneous tumor regression. The main immunodominant epitopes of carbonic anhydrase isoform I (CA I) have previously been identified using epitope extraction technique in combination with mass spectrometric detection and bioinformatic verification.

A new species of the genus Duvalius sg. Neoduvalius from Montenegro with taxonomical remarks on the genus Duvalius (Coleoptera, Carabidae, Trechini).

Duvalius (sg. Neoduvalius) gejzadunayi sp. n.

Antibodies against carbonic anhydrase in patients with aplastic anemia.

Background/aims: Antibodies against carbonic anhydrase (CA) have been detected in patients with an aplastic anemia (AA)-like syndrome after autologous stem cell transplantation.

Methods: We analyzed sera of 53 bona fide AA patients before and after treatment with anti-thymocyte globulin (ATG) or bone marrow transplantation for the presence of anti-CA antibodies.

Results: Anti-CA antibodies were detected in 20 patients (38%) and were associated with older age at diagnosis of AA.

Tandem autologous stem cell transplantation in multiple myeloma after high-dose chemotherapy with two separate collections: single institution experience.

Presented is a retrospective analysis of multiple myeloma patients transplanted at our institution between November 1993 and August 2007. The objective of this analysis was to assess the feasibility and toxicity of tandem autologous stem cell transplantation (T-ASCT) when stem cells were harvested before first and before second transplantation separately. A total of 90 patients transplanted in our center were analyzed, of whom 43 patients were in tandem transplantation group.