Combined NMR and MS-based metabonomics and real-time PCR analyses reveal dynamic metabolic changes of Ganoderma lucidum during fruiting body growing.

Ganoderma lucidum (G. lucidum) is a rare medicinal fungus with various beneficial properties. One of its main components, ganoderic acids (GAs), are important triterpenoids known for their sedative and analgesic, hepatoprotective, and anti-tumor activities.

The Different Metabolic Responses of Resistant and Susceptible Wheats to Inoculation.

Fusarium head blight (FHB) is a serious wheat disease caused by Fusarium graminearum () Schwabe. FHB can cause huge loss in wheat yield. In addition, trichothecene mycotoxins produced by are harmful to the environment and humans.

Effects of miR-211-3p/RHBDD1 axis on cell proliferation, cell cycle progression, and epithelial-mesenchymal transition in glioma.

This study was designed to elucidate the relationship of miR-211-3p and rhomboid domain containing 1 (RHBDD1) in glioma. Here, we first observed that miR-211-3p directly targets the 3˘-UTR of RHBDD1 in glioma cells using dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and Western blot analysis. Pearson's correlation analysis showed that miR-211-3p expression was negatively correlated with RHBDD1 expression in glioma tissues.

H NMR and UHPLC/Q-Orbitrap-MS-Based Metabolomics Combined with 16S rRNA Gut Microbiota Analysis Revealed the Potential Regulation Mechanism of Nuciferine in Hyperuricemia Rats.

Hyperuricemia seriously jeopardizes human health by increasing the risk of several diseases, such as gout and stroke. Nuciferine is able to alleviate hyperuricemia significantly. However, the underlying metabolic regulation mechanism remains unknown.

Metabolic Changes of Induced by Gene Deletion.

Fusarium head blight (FHB) mainly resulting from () Schwabe is a notorious wheat disease causing huge losses in wheat production globally. also produces mycotoxins, which are harmful to human and domestic animals. In our previous study, we obtained two mutants, and , respectively, with a single deletion of trehalose 6-phosphate synthase () and trehalose 6-phosphate phosphatase () compared with the wild type (WT).

Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages.

Background: Mycobacterium tuberculosis is the major pathogen of tuberculosis, which affects approximately one-third of the world's population. The 6 kDa early secreted antigenic target (ESAT6) and the 10 kDa culture filtrate protein (CFP10), which are secreted by the ESX-1 system in M. tuberculosis, can contribute to mycobacterial virulence.