Generation and Characterization of a Dual-Reporter Transgenic Line Expressing eGFP and Luciferase.

In this study, we generated a transgenic strain of , an etiological agent associated with a diversity of clinical manifestations of leishmaniasis ranging from localized cutaneous to mucocutaneous to disseminated disease. Transgenic parasites expressing reporter proteins are valuable tools for studies of parasite biology, host-pathogen interactions, and anti-parasitic drug development. To this end, we constructed an line stably expressing the reporters eGFP and luciferase (eGFP-LUC .

The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Infection .

Leishmaniasis is an infectious disease caused by protozoans of the genus . The macrophage is the resident cell in which the parasite replicates and it is important to identify new compounds that can aid in parasite elimination since the drugs used to treat leishmaniasis are toxic and present side effects. We have previously shown that treatment of -infected macrophages with DETC (Diethyldithiocarbamate) induces parasite killing, .