Publications by authors named "Lais Papini Fernandes"
Evaluation of immunohistochemical expression of p16 and presence of human papillomavirus in oral and oropharyngeal carcinoma.
Ana Luíza Laguardia Cantarutti Lais Papini Fernandes Marina Vaz de Lima Fullin Saldanha Ana Elizia Mascarenhas Marques Leonora Maciel de Souza Vianna
J Craniofac Surg
January 2014
Article Synopsis
- Carcinogenesis involves the inactivation of tumor suppressor genes like p16 and the activation of protooncogenes, leading to uncontrolled cell growth; p16 inactivation occurs early in many squamous cell carcinoma cases.
- The study aimed to analyze p16 protein expression in oral and oropharyngeal squamous cell carcinoma, involving examination of tumor tissues, surgical margins, and potential links to human papillomavirus (HPV).
- Results showed that p16 expression was low in tumor areas (38.46%) but higher in normal and dysplastic margins; however, no association was found between p16 expression and HPV presence, as none of the patients tested positive for HPV.
Collecting buccal cell DNA has a number of advantages in terms of practicality and technical quality when employed for detecting human papillomavirus (HPV), arguably an important etiological factor for head and neck carcinomas. The objective of the present study was to assess oropharyngeal mucosa brushing as an effective method for collecting DNA samples and for detecting HPV. Forty-three female patients diagnosed with cervical intraepithelial neoplasia, and 21 of their spouses, both groups without clinically detectable oral lesions, were submitted to quantitative and qualitative DNA analysis obtained through buccal cell brushing, with spectrophotometry assessment and beta-globin PCR.
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