Characterization of specific Trichomonas vaginalis target antigens from different isolates by immunoblotting against hyperimmune rabbit serum.

The SDS-PAGE and immunoblot methods were used to identify Trichomonas vaginalis specific target antigen(s). Ten T. vaginalis isolates, cultured on TYM media, were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE), revealed 34 distinct bands.

Growth patterns and antigenic analysis of Egyptian Trichomonas vaginalis isolates.

The vaginal washouts from symptomatic and asymptomatic patients were examined by wet mount examination and culture on modified TYM medium. Among the 320 cases examined, 10 were positive for T. vaginalis trophozoites by wet mount examination and culture.