Bilayered Medium for Rapid Isolation of Mycobacterium tuberculosis.

Background: A bilayered medium (BLM) seemed to indicate a rapid recovery time from Mycobacterium tuberculosis (M. tuberculosis) for the patients in question, namely, 48 hours.

Method: Sputum specimens collected from 112 patients with clinically suspected pulmonary tuberculosis were subjected to Ziehl-Neelsen staining and inoculation on Löwenstein-Jensen medium (LJM) and BLM.