Outbreak Investigation and Molecular Detection of Pox Virus Circulating in Sheep and Goats in Selected Districts of West Gojjam and Awi Zones Northwest, Ethiopia.

Introduction: Sheep and goat pox virus (SGPV) is a systemic contagious disease causing extreme illness and death in small ruminants.

Methods: A cross-sectional study was conducted in West Gojjam and Awi zone of Amhara national regional state Northwest Ethiopia, from November 2018 to May 2019 with the objective of pox virus outbreak investigation and molecular detections in sheep and goats (shoats). The study included clinical examinations of lesions, laboratory analysis, and questionnaire survey.

Effect of Feeding Improved Grass Hays and Straw Silage on Milk Yield, Nitrogen Utilization, and Methane Emission of Lactating Fogera Dairy Cows in Ethiopia.

The nutritionally imbalanced poor-quality diet feeding is the major constraint of dairy production in tropical regions. Hence, alternative high-quality roughage-based diets are required to improve milk yield and reduce methane emission (CH4). Thus, we tested the effects of feeding natural pasture hay, improved forage grass hays (Napier and Hybrid), and treated crop residues ( straw) on nutrient digestibility, milk yield, nitrogen balance, and methane emission.

Aptamer-mediated inhibition of Mycobacterium tuberculosis polyphosphate kinase 2.

Inorganic polyphosphate (polyP) plays a number of critical roles in bacterial persistence, stress, and virulence. PolyP intracellular metabolism is regulated by the polyphosphate kinase (PPK) protein families, and inhibition of PPK activity is a potential approach to disrupting polyP-dependent processes in pathogenic organisms. Here, we biochemically characterized Mycobacterium tuberculosis (MTB) PPK2 and developed DNA-based aptamers that inhibit the enzyme's catalytic activities.