Stimulus-dependent neuronal cell responses in SH-SY5Y neuroblastoma cells.

The aim of the present study was to elucidate the intracellular mechanisms that cause neuronal cell death following exposure to excitatory neurotransmitter‑induced neurotoxicity, neurotoxins and oxidative stress. Human SH‑SY5Y neuroblastoma cells were exposed to various stimuli, including glutamate, 6‑hydroxydopamine (6‑OHDA), and glucose oxidase, and cell viability was determined by MTT assay. Early apoptosis and necrosis were examined by Annexin V/propidium iodide double staining and flow cytometric analysis.