Molecular characterization of neuropeptide Y gene in Chinese perch, an acanthomorph fish.

The full-length neuropeptide Y (NPY) cDNA of Chinese perch Siniperca chuatsi was 704 bp in length, and contained a 300 bp open reading frame encoding a prepro-NPY with 99 amino acids. The predicted prepro-NPY peptide contained a putative signal peptide of 28 amino acids and a mature NPY of 36 amino acids, followed by the proteolytic processing site Gly-Lys-Arg and 35 amino acids that comprise the C-terminal peptide of NPY. Amino acid alignment and phylogenetic analysis indicate that the predicted Chinese perch prepro-NPY (composed of 99 amino acids) had high identities to the prepro-NPY of acanthomorph fishes (93-95%), whereas it had much lower identities to the prepro-NPY (composed of 96 or 97 amino acids) of cyprinid fishes (59-60%) or mammals (57-63%).